Identification of molecular interactions between P-site tRNA and the ribosome essential for translocation
Identification of molecular interactions between P-site tRNA and the ribosome essential for translocation
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DOI：
10.1073/pnas.211184098
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发表时间：
2001-09-25
影响因子：
11.1
通讯作者：
Joseph, S
中科院分区：
文献类型：
--
作者：
Feinberg, JS;Joseph, S
tRNA-mRNA 复合物的易位是蛋白质合成延伸循环中的基本步骤。我们的研究表明，核糖体可以通过在 T psiC 环中位置 56 和 57 之间的磷酸二酯主链断裂来易位 P 位点结合的 tRNA(Met)。我们使用这种片段化的 P 位点结合的 tRNAMet 来鉴定 3' 受体臂中位置 71 和 76 处的两个 2' -羟基，这对于易位至关重要。晶体学数据显示，位置71和76处的2'-羟基分别接触核糖体E位点中235个rRNA残基1892和2433-2434的主链。这些结果建立了 P 位点 tRNA 和 235 rRNA 之间的一组功能相互作用，这对于易位至关重要。
Translocation of the tRNA-mRNA complex is a fundamental step in the elongation cycle of protein synthesis. Our studies show that the ribosome can translocate a P-site-bound tRNA(Met) with a break in the phosphodiester backbone between positions 56 and 57 in the T psiC-loop. We have used this fragmented P-site-bound tRNAMet to identify two 2 ' -hydroxyl groups at positions 71 and 76 in the 3 ' -acceptor arm that are essential for translocation. Crystallographic data show that the 2 ' -hydroxyl group at positions 71 and 76 contacts the backbone of 235 rRNA residues 1892 and 2433-2434, respectively, in the ribosomal E site. These results establish a set of functional interactions between P-site tRNA and 235 rRNA that are essential for translocation.