Use of Cognitive Testing, Questionnaires, and Plasma Biomarkers to Quantify Cognitive Impairment in an Aging Pet Dog Population.
Use of Cognitive Testing, Questionnaires, and Plasma Biomarkers to Quantify Cognitive Impairment in an Aging Pet Dog Population.
复制标题
DOI：
10.3233/jad-215562
复制
发表时间：
2022
影响因子：
4
通讯作者：
Gruen, Margaret E.
中科院分区：
文献类型：
--
作者：
Fefer, Gilad;Panek, Wojciech K.;Khan, Michael Z.;Singer, Matthew;Westermeyer, Hans D.;Mowat, Freya M.;Murdoch, David M.;Case, Beth;Olby, Natasha J.;Gruen, Margaret E.
关键词：
老龄犬患有认知功能障碍综合征（CCDS），这是一种认知能力下降与淀粉样蛋白病理学和皮质萎缩相关的疾病。推定诊断是通过身体检查，排除全身/代谢疾病和完成筛选问卷的主人。本研究旨在确定老年宠物犬的认知功能是否可以量化，并将认知测试与经验证的问卷和老年犬的血浆神经丝轻链（pNfL）浓度相关联。招募了来自15个品种的39只犬（9.3至15.3岁）。犬主人完成了犬痴呆量表（CADES）和犬认知功能障碍评定量表（CCDR）。执行控制和社会线索进行了测试，并与单分子阵列测定pNfL。比较认知测试评分、CADES、CCDR评分和pNfL。CADES评分将5只犬分类为重度CCDS，6只为中度，10只为轻度，18只为正常。CCDR确定7只犬存在CCDS风险，32只正常。大多数狗都可以进行认知测试，尽管严重受影响的狗无法学习任务。CADES评分与持续注意力持续时间（r=-0.47，p=0.002）、抑制控制（r=-0.51，p=0.002）、迂回（r=-0.43，p=0.001）和pNfL（r=0.41，p=0.025）相关。pNfL浓度与抑制对照相关（r=-0.7，p=<0.001）。CCDR量表与抑制控制的表现相关（r=-0.46，p=0.005）。我们的研究结果表明，使用问卷调查，特定的认知测试和pNfL浓度相结合的多维方法可用于量化老化宠物狗的认知下降。
Aging dogs suffer from Cognitive Dysfunction Syndrome (CCDS), a condition in which cognitive decline is associated with amyloid pathology and cortical atrophy. Presumptive diagnosis is made through physical examination, exclusion of systemic/metabolic conditions and completion of screening questionnaires by owners. This study aimed to determine whether cognitive function could be quantified in aging pet dogs, and to correlate cognitive testing with validated questionnaires and plasma neurofilament light chain (pNfL) concentration in aging dogs. Thirty-nine dogs from fifteen breeds were recruited (9.3 to 15.3 years). Owners completed the Canine Dementia Scale (CADES), and Canine Cognitive Dysfunction Rating scale (CCDR). Executive control and social cues were tested and pNfL was measured with single molecule array assay. Comparisons were made between cognitive testing scores, CADES, CCDR scores, and pNfL. CADES scoring classified five dogs as severe CCDS, six as moderate, ten as mild and eighteen as normal. CCDR identified seven dogs at risk of CCDS and thirty-two as normal. Cognitive testing was possible in the majority of dogs, although severely affected dogs were unable to learn tasks. CADES score correlated with sustained attention duration (r=−0.47, p=0.002), inhibitory control, (r=−0.51, p=0.002), detour, (r=−0.43, p=0.001), and pNfL (r=0.41, p=0.025). Concentration of pNfL correlated with inhibitory control (r=−0.7, p=<0.001). The CCDR scale correlated with performance on inhibitory control (r=−0.46, p=0.005). Our findings suggest that a multi-dimensional approach using a combination of questionnaires, specific cognitive tests and pNfL concentration can be used to quantify cognitive decline in aging pet dogs.