A mouse model of vitiligo based on endogenous auto-reactive CD8 + T cell targeting skin melanocyte.
A mouse model of vitiligo based on endogenous auto-reactive CD8 + T cell targeting skin melanocyte.
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DOI：
10.1186/s13619-022-00132-9
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发表时间：
2022-10-02
影响因子：
--
通讯作者：
Chen, Ting
中科院分区：
文献类型：
--
作者：
Chen, Daoming;Xu, Zijian;Cui, Jun;Chen, Ting
白癜风是人类最常见的皮肤色素脱失性疾病。它由破坏皮肤黑素细胞的内源性自身反应性CD 8 + T细胞介导。据估计，这种疾病的患病率为全球人口的1%，目前尚无治愈方法。动物模型是理解白癜风发病机制和开发新疗法不可或缺的工具。在这里，我们描述了一种白癜风小鼠模型，它概括了白癜风的关键临床特征，包括表皮色素脱失，皮肤中的CD 8 + T细胞浸润和黑素细胞丢失。为了激活靶向黑素细胞的内源性自身反应性细胞毒性CD 8 + T细胞，该模型依赖于B16 F10黑色素瘤细胞的瞬时接种和CD 4+调节性T细胞的耗竭。在细胞水平，表皮CD 8 + T细胞浸润和黑素细胞损失早在治疗后第19天开始。2个月后出现肉眼可见的表皮色素脱失。该方案可仅使用市售试剂在任何C57 BL/6背景小鼠品系中有效诱导白癜风。这使研究人员能够利用小鼠遗传学工具进行深入的体内白癜风研究，并为药物发现提供了强大的平台。
Vitiligo is the most common human skin depigmenting disorder. It is mediated by endogenous autoreactive CD8 + T cells that destruct skin melanocytes. This disease has an estimated prevalence of 1% of the global population and currently has no cure. Animal models are indispensable tools for understanding vitiligo pathogenesis and for developing new therapies. Here, we describe a vitiligo mouse model which recapitulates key clinical features of vitiligo, including epidermis depigmentation, CD8 + T cell infiltration in skin, and melanocyte loss. To activate endogenous autoreactive cytotoxic CD8 + T cells targeting melanocytes, this model relies on transient inoculation of B16F10 melanoma cells and depletion of CD4 + regulatory T cells. At cellular level, epidermal CD8 + T cell infiltration and melanocyte loss start as early as Day 19 after treatment. Visually apparent epidermis depigmentation occurs 2 months later. This protocol can efficiently induce vitiligo in any C57BL/6 background mouse strain, using only commercially available reagents. This enables researchers to carry out in-depth in vivo vitiligo studies utilizing mouse genetics tools, and provides a powerful platform for drug discovery.
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影响因子：
30.8
作者：
Jin, Ying;Andersen, Genevieve;Yorgov, Daniel;Ferrara, Tracey M.;Ben, Songtao;Brownson, Kelly M.;Holland, Paulene J.;Birlea, Stanca A.;Siebert, Janet;Hartmann, Anke;Lienert, Anne;van Geel, Nanja;Lambert, Jo;Luiten, Rosalie M.;Wolkerstorfer, Albert;van der Veen, J. P. Wietze;Bennett, Dorothy C.;Taieb, Alain;Ezzedine, Khaled;Kemp, E. Helen;Gawkrodger, David J.;Weetman, Anthony P.;Koks, Sulev;Prans, Ele;Kingo, Kulli;Karelson, Maire;Wallace, Margaret R.;McCormack, Wayne T.;Overbeck, Andreas;Moretti, Silvia;Colucci, Roberta;Picardo, Mauro;Silverberg, Nanette B.;Olsson, Mats;Valle, Yan;Korobko, Igor;Boehm, Markus;Lim, Henry W.;Hamzavi, Iltefat;Zhou, Li;Mi, Qing-Sheng;Fain, Pamela R.;Santorico, Stephanie A.;Spritz, Richard A.
通讯作者：
Spritz, Richard A.
影响因子：
4.3
作者：
Eby JM;Kang HK;Klarquist J;Chatterjee S;Mosenson JA;Nishimura MI;Garrett-Mayer E;Longley BJ;Engelhard VH;Mehrotra S;Le Poole IC
通讯作者：
Le Poole IC
影响因子：
158.5
作者：
Jin, Ying;Mailloux, Christina M.;Spritz, Richard A.
通讯作者：
Spritz, Richard A.
影响因子：
3.6
作者：
Hamidizadeh, Nasrin;Ranjbar, Sara;Handjani, Farhad
通讯作者：
Handjani, Farhad
影响因子：
10.9
作者：
Hua, Camille;Boussemart, Lise;Robert, Caroline
通讯作者：
Robert, Caroline