Voltage-dependent potassium channels in cytokeratin-positive and cytokeratin-negative microvascular endothelial cells of the corpus luteum
Voltage-dependent potassium channels in cytokeratin-positive and cytokeratin-negative microvascular endothelial cells of the corpus luteum
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DOI：
10.1007/s00441-001-0501-z
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发表时间：
2002-10-01
影响因子：
3.6
通讯作者：
Nieber, K
中科院分区：
文献类型：
--
作者：
Richter, M;Tscheudschilsuren, G;Nieber, K
我们最近描述了细胞角蛋白阳性（CK+）和细胞角蛋白阴性（CK-）微血管内皮细胞（MVECs）在牛黄体。两种表型显示粘附分子和细胞因子的不同表达和释放。由于介质的分泌是由电生理膜参数具体调节，本报告将比较在相同条件下培养的两种细胞类型中的电压依赖性钾（K+）通道。来源于同一器官微血管床的CK+和CK-MVECs的K+通道特性不同.在CK-MVEC中，发现了一个内向整流的K+电流，显示了Kir2.1的特征. CK+ MVEC显示电压依赖性K+电流，其在去极化时快速激活，并且非常缓慢地失活，并且与Kv家族的成员相关。RT-PCR检测到CK-和CK+ MVECs中Kir2.1的mRNA表达，但没有Kv1.4 mRNA的证据。Kir2.1在CK+ MVECs中的功能可能在培养过程中被诱导，而CK+ MVECs似乎对环境条件更具抗性，并且不表达内向电流。
We have recently described cytokeratin-positive (CK+) and cytokeratin-negative (CK-) microvascular endothelial cells (MVECs) in the bovine corpus luteum. The two phenotypes show a different expression and release of adhesion molecules and cytokines. Since secretion of mediators is specifically regulated by the electrophysiological membrane parameters, this report will compare voltage-dependent potassium (K+) channels in the two cell types cultured under the same conditions. CK+ and CK- MVECs derived from the microvascular bed of one organ differ in their K+-channel characteristics. In CK- MVECs, an inwardly rectifying K+ current was discovered, showing the characteristics of the Kir2.1. CK+ MVECs displayed a voltage-dependent K+ current that activates rapidly on depolarization and inactivates very slowly, and is associated with a member of the Kv family. The mRNA for Kir2.1 was identified by RT-PCR in CK- and CK+ MVECs, but there was no evidence of Kv1.4 mRNA in either of them. The function of Kir2.1 in CK+ MVECs might be induced during cultivation, whereas CK+ MVECs appear to be more resistant to environmental conditions and do not express an inward current.