Bilateral Cortical Hyperactivity Ditected by fMRI Associates with Improved Motor Function Following Intravenous Infusion of Mesenchymal Stem Cells in a Rat Stroke Model
Bilateral Cortical Hyperactivity Ditected by fMRI Associates with Improved Motor Function Following Intravenous Infusion of Mesenchymal Stem Cells in a Rat Stroke Model
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在大鼠中风模型中静脉输注间充质干细胞后，fMRI 检测到双侧皮质过度活跃与运动功能改善相关
DOI：
10.1016/j.brainres.2012.12.028
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Suzuki J
中科院分区：
文献类型：
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作者：
菱川朋人;杉生憲志;平松匡文;春間 純;高杉祐二;新治有径;西廣真吾;伊達 勲;坂井信幸;Suzuki J
骨髓间充质干细胞静脉移植改善大鼠脑梗死模型的功能障碍。在本研究中，在大鼠右侧大脑中动脉闭塞（MCAO）诱导后6h静脉内施用MSC。在MCAO后第1、4、7、14、21、28和42天进行电刺激左前爪期间的功能MRI（fMRI）和行为测试（跑台应激测试）。在介质注入组（n=20），电刺激左前爪引起单侧（右皮层）激活信号检测梗死的躯体感觉皮层的fMRI。在MSC输注的动物中，观察到两种fMRI模式：单侧（n=17）和双侧（n=19）感觉运动皮层的激活。在MSC组中，单侧和双侧皮质激活的动物与培养基输注组相比显示出显著改善的运动功能。然而，MSC组的双侧激活模式显示出最大的功能恢复。与中等组相比，MSC组中使用T2WI从高强度信号计算的病变体积较小，但MSC组中单侧和双侧信号的病变体积相同。这些结果表明，通过功能磁共振成像检测到的感觉运动皮层中的双侧信号的存在比单独的损伤体积更能预测功能结果的改善。
Intravenous transplantation of mesenchymal stem cells (MSCs) derived from bone marrow ameliorates functional deficits in rat cerebral infarction models. In this study, MSCs were intravenously administered 6h after right middle cerebral artery occlusion (MCAO) induction in rat. Functional MRI (fMRI) during electrical stimulation of the left forepaw and behavioral testing (treadmill stress test) were carried out at day 1, 4, 7, 14, 21, 28 and 42 following MCAO. In medium infused group (n=20) electrical stimulation of the left forepaw elicited a unilateral (right cortex) activated signal detected by fMRI in the infarcted somatosensory cortex. In the MSC infused animals two fMRI patterns were observed: unilateral (n=17) and bilateral (n=19) activation of sensorimotor cortex. In the MSC group both unilateral and bilateral cortical activated animals displayed significantly improved motor function compared to the medium infused group. However, the bilateral activated pattern in the MSC group showed the greatest functional recovery. Lesion volume as calculated from high intensity signals using T2WI was less in the MSC groups as compared to the medium group, but the lesion volume for the unilateral and bilateral signals in the MSC group was the same. These results suggest that the presence of a bilateral signal in sensorimotor cortex as detected by fMRI was more predictive of improved functional outcome than lesion volume alone.