Rechargeable adhesive with calcium phosphate nanoparticles inhibited long-term dentin demineralization in a biofilm-challenged environment
Rechargeable adhesive with calcium phosphate nanoparticles inhibited long-term dentin demineralization in a biofilm-challenged environment
复制标题
含有磷酸钙纳米粒子的可充电粘合剂在生物膜挑战的环境中抑制长期牙本质脱矿
DOI：
10.1016/j.jdent.2020.103529
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发表时间：
2021
影响因子：
4.4
通讯作者：
Li Jiyao
中科院分区：
文献类型：
--
作者：
Yu Zhaohan;Tao Siying;Xu Hockin H. K.;Weir Michael D.;Fan Menglin;Liu Yifang;Zhou Xuedong;Liang Kunneng;Li Jiyao
目的研究纳米无定形磷酸钙(NACP)粘接剂在生物膜环境中对牙本质的长期脱矿抑制能力。方法将NACP粘结剂浸泡在pH为4的溶液中，排出钙(Ca)和磷(P)离子，然后用Ca和P离子充电。将牙本质标本在变形链球菌生物膜下脱矿24 h，随机分为两组：(1)牙本质对照组；(2)牙本质再充NACP粘结剂组。所有标本均在含1%蔗糖(BHIS)的脑心输液中浸泡4 h，然后加入人工唾液(AS)20 h，重复10 d。每日测定BHIS的pH值、BHIS和AS中的钙、磷离子含量。10d后，对生物膜的乳酸产量和菌落形成单位进行检测。结果对照组牙本质进一步脱矿。充电后的NACP粘合剂中和酸，将pH提高到5以上，每天释放大量的钙和磷离子。在生物膜挑战环境中，可充电的NACP粘结剂可减少乳酸的产生(P <； 0.05)，抑制牙本质脱矿，维持牙本质硬度，具有良好的长期脱矿抑制能力。结论在生物膜挑战环境中，可充电的NACP粘结剂可通过对钙、磷离子的补充，持续抑制牙本质脱矿。
ObjectivesThis study aims to investigate the long-term demineralization-inhibition capability of a rechargeable adhesive with nanoparticles of amorphous calcium phosphate (NACP) on dentin in a biofilm-challenged environment.MethodsThe NACP adhesive was immersed in a pH 4 solution to exhaust calcium (Ca) and phosphate (P) ions and then recharged with Ca and P ions. Dentin samples were demineralized underStreptococcus mutans biofilms for 24 h and randomly divided into two groups: (1) dentin control, (2) dentin with recharged NACP adhesives. Each day, all the samples were immersed in brain heart infusion broth with 1% sucrose (BHIS) for 4 h, and then in artificial saliva (AS) for 20 h. This cycle was repeated for 10 days. The pH of BHIS, the Ca and P ions content of the BHIS and AS were measured daily. After 10 days, the lactic acid production and colony-forming units of the biofilms were tested. The changes of remineralization/demineralization were also analyzed.ResultsDentin in the control group showed further demineralization. The recharged NACP adhesive neutralized acids, increasing the pH to above 5, and released large amounts of Ca and P ions each day. The recharged NACP adhesive decreased the production of lactic acid (P < 0.05), inhibited dentin demineralization and sustained the dentin hardness in the biofilm-challenged environment, showing an excellent long-term demineralization-inhibition capability.ConclusionsThe NACP adhesive could continuously inhibit dentin demineralization in a biofilm-challenged environment by recharging with Ca and P ions.SignificanceThe rechargeable NACP adhesive could provide long-term dentin bond protection.