Thermal effect on dispersive infrared spectroscopic imaging of prostate cancer tissue
Thermal effect on dispersive infrared spectroscopic imaging of prostate cancer tissue
复制标题
前列腺癌组织色散红外光谱成像的热效应
DOI：
10.1002/jbio.201800187
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发表时间：
2018
影响因子：
2.8
通讯作者：
Kazarian Sergei G.
中科院分区：
文献类型：
--
作者：
Song Cai L.;Ryu Meguya;Morikawa Junko;Kothari Archana;Kazarian Sergei G.
开发了一种结合色散红外显微光谱成像和热成像的系统来研究热辐射对前列腺活检样本红外吸收光谱的影响。该系统允许根据光谱成像获得的积分吸光度来解释作为发射率函数的热信号强度分布。光谱中可以识别样本内癌症和良性区域之间的生化差异。 H&E 染色的邻近组织切片与去除热效应前后构建的红外图像的并排比较表明，后者强烈支持组织内区域的分化。在离散波长处使用光谱带显着减少了光谱采集时间，使得该技术有望成为未来的临床诊断工具。采用系统方法来处理数据，首先在没有先验知识的情况下对二阶导数谱进行 k 均值聚类，然后进行主成分分析 (PCA)。根据亚甲基官能团的反对称拉伸模式，成功地对组织样本内的四个不同区域进行了分类。当将光谱投影到由前 2 个主成分构成的平面上的 PCA 得分图上时，簇中的数据之间会发生分离。通过统计检验验证了差异的显着性。调节斩波器和检测器的信号可以同时采集前列腺活检组织的红外和热图像。
A system that combines dispersive infrared micro‐spectroscopic imaging and thermography has been developed to study the effect of thermal radiation on the infrared absorption spectra of prostate biopsy samples. The system allows the distribution of thermal signal intensity as a function of emissivity to be interpreted from the integrated absorbance obtained by spectroscopic imaging. Biochemical differences between cancer and benign areas within the specimens are identified in the spectra. Side‐by‐side comparison of H&E stained adjacent tissue sections with infrared images constructed before and after the removal of thermal effect showed that the latter strongly support differentiation of regions within tissues. The use of spectral bands at discrete wavelengths significantly reduced spectral acquisition time, making this technique promising as a future clinical diagnostic tool. A systemic methodology was implemented to process the data, first by k‐means clustering on the second derivative spectra without a priori knowledge, followed by principal component analysis (PCA). Four distinct regions within the tissue samples were successfully classified based on the antisymmetric stretching mode of the methylene functional group. Separation between data in clusters occurs when projecting spectra on a PCA score plot on a plane made by first 2 principal components. The significance of the disparity was verified with statistical test. Regulation of signal to chopper and detector enables simultaneous acquisition of infrared and thermal images of the prostate biopsy tissues.