Behavioral, histological, and ex vivo magnetic resonance imaging assessment of graded contusion spinal cord injury in mice
Behavioral, histological, and ex vivo magnetic resonance imaging assessment of graded contusion spinal cord injury in mice
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DOI：
10.1089/neu.2006.0204
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发表时间：
2007-04-01
影响因子：
4.2
通讯作者：
Anderson, Aileen J.
中科院分区：
文献类型：
--
作者：
Nishi, Rebecca A.;Liu, Hongli;Anderson, Aileen J.
本研究描述了用于小鼠挫伤性脊髓损伤（SCI）模型的Infinite Horizon（IH）撞击器的特征，并研究了使用磁共振成像（MRI）作为精确测量小鼠挫伤性SCI后病变体积的方法的可行性和可靠性。8周龄雌性C57 B1/6小鼠接受轻度（30千达因）、中度（50千达因）或重度（70千达因）挫伤：T9椎骨水平的损伤。未受伤的对照小鼠仅接受T9椎板切除术。使用Basso、Beattie、Bresnahan（BBB）和Basso小鼠量表（BMS）旷场运动评定量表评估功能恢复。接下来，在T6和T12脊神经根之间收集4%多聚甲醛灌注的脊髓，并在4 ℃下储存在磷酸盐缓冲盐水（PBS）中直至MRI分析。使用7特斯拉MRI上的T1加权图像确定MRI病变体积。在从相同脊髓加工的20 μ m聚酯蜡包埋切片上进行组织学检查，用于纤维连接蛋白损伤体积和髓鞘碱性蛋白保留的白色物质体积的体视学测定。还分析了震中的备用白色物质面积。结果表明，IH冲击器在小鼠中产生精确的分级挫伤SCI。损伤体积与撞击力呈正相关，与保留的白色物质和功能恢复呈负相关。此外，使用纤连蛋白染色和MRI分析检测到相似的病变体积，尽管MRI可能对轻度损伤更敏感。这些结果为研究人员在如何分析动物模型中的脊髓损伤提供了更多选择。
This study characterized the Infinite Horizon (IH) Impactor for use in mouse models of contusion spinal cord injury (SCI), and investigated the feasibility and reliability of using magnetic resonance imaging (MRI) as a method to accurately measure lesion volume after mouse contusion SCI. Eight-week-old female C57Bl/6 mice received a mild (30 kilodyne), moderate (50 kilodyne), or severe (70 kilodyne) contusion: injury at the T9 vertebral level. Uninjured control mice received a T9 laminectomy only. Functional recovery was assessed using the Basso, Beattie, Bresnahan (BBB) and Basso Mouse Scale (BMS) open-field locomotor rating scales. Next, 4% paraformaldehyde-perfused spinal cords were collected between the T6 and T12 spinal roots, and stored in phosphate-buffered saline (PBS) at 4 degrees C until MRI analysis. MRI lesion volumes were determined using T1-weighted images on a 7-Tesla MRI. Histology was performed on 20-mu m polyester wax-embedded sections processed from the same spinal cords for stereological determination of fibronectin lesion volume and myelin basic protein spared white matter volume. Area of spared white matter at the epicenter was also analyzed. The results demonstrated that the IH Impactor produced precise, graded contusion SCI in mice. Lesion volumes were positively correlated with force of impact, and negatively correlated with spared white matter and functional recovery. Additionally, similar lesion volumes were detected using fibronectin staining and MRI analysis, although MRI may be more sensitive for milder injuries. These results give researchers more options in how to analyze spinal cord injuries in animal models.