A DOUBLE-STRANDED-RNA UNWINDING ACTIVITY INTRODUCES STRUCTURAL ALTERATIONS BY MEANS OF ADENOSINE TO INOSINE CONVERSIONS IN MAMMALIAN-CELLS AND XENOPUS EGGS
A DOUBLE-STRANDED-RNA UNWINDING ACTIVITY INTRODUCES STRUCTURAL ALTERATIONS BY MEANS OF ADENOSINE TO INOSINE CONVERSIONS IN MAMMALIAN-CELLS AND XENOPUS EGGS
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DOI：
10.1073/pnas.86.8.2647
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发表时间：
1989-04-01
影响因子：
11.1
通讯作者：
NISHIKURA, K
中科院分区：
文献类型：
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作者：
WAGNER, RW;SMITH, JE;NISHIKURA, K
据报道，两栖动物的卵和胚胎以及哺乳动物的细胞都含有一种能解开双链RNA的活性物质。我们现在已经发现，腺苷残基已被修改的RNA产品的这种解旋活动。虽然修饰后的RNA仍然是双链的，但这种修饰导致RNA对单链特异性RNA酶敏感，并在天然聚丙烯酰胺电泳凝胶上以延迟涂片的形式迁移。该修饰对双链RNA具有特异性。在给定的随机序列RNA分子中，至少40%的腺苷残基可以在体外被修饰。通过使用标准的二维TLC和HPLC分析，修饰的碱已被鉴定为肌苷。肌苷和尿苷之间的碱基配对不匹配似乎是负责观察到的特征的解绕RNA。讨论了这种修饰活性和修饰的双链RNA的生物学意义。
Amphibian eggs and embryos as well as mammalian cells have been reported to contain an activity that unwinds double-stranded RNA. We have now found that adenosine residues have been modified in the RNA products of this unwinding activity. Although the modified RNA remains double-stranded, the modification causes the RNA to be susceptible to single-strand-specific RNase and to migrate as a retarded smear on a native polyacrylamide electrophoresis gel. The modification is specific for double-stranded RNA. At least 40% of the adenosine residues can be modified in vitro in a given random sequence RNA molecule. By using standard two-dimensional TLC and HPLC analyses, the modified base has been identified as inosine. Mismatched base-pairing between inosine and uridine appears to be responsible for the observed characteristics of the unwound RNA. The biological significance of this modifying activity and also of the modified double-stranded RNA is discussed.