Identification and expression in mouse of two heparan sulfate glucosaminyl N-deacetylase/N-sulfotransferase genes
Identification and expression in mouse of two heparan sulfate glucosaminyl N-deacetylase/N-sulfotransferase genes
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DOI：
10.1074/jbc.273.19.11902
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发表时间：
1998-05-08
影响因子：
4.8
通讯作者：
Kjellén, L
中科院分区：
文献类型：
--
作者：
Kusche-Gullberg, M;Eriksson, I;Kjellén, L
硫酸肝素/肝素的生物合成是一个复杂的过程，需要许多不同酶的协调作用。与多糖链聚合密切相关的修饰反应是由n -去乙酰化引发的，然后是n -乙酰氨基氨基的n -磺化。这两个反应是由一个蛋白质完成的。首次从大鼠肝脏和小鼠肥大细胞瘤中纯化并克隆出具有这种双重活性的蛋白。小鼠肥大细胞瘤酶由类似于4千碱基（kb）的mRNA编码，而大鼠肝脏转录物含有类似于8 kb的mRNA。在本研究中，描述了小鼠8-kb转录本编码的酶的初级结构。结果表明，4- kb和8-kb转录本具有广泛的组织分布，并由不同的基因编码。编码4kb转录本的基因表征表明，它跨越约8kb的区域，并且包含至少14个外显子。讨论了该基因与先前鉴定的8 kb转录本的人类基因的相似性。
The biosynthesis of heparan sulfate/heparin is a complex process that requires the coordinate action of a number of different enzymes. In close connection with polymerization of the polysaccharide chain, the modification reactions are initiated by N-deacetylation followed by N-sulfation of N-acetylglucosamine units. These two reactions are carried out by a single protein. Proteins with such dual activities were first purified and cloned from rat liver and mouse mastocytoma. The mouse mastocytoma enzyme is encoded by an similar to 4-kilobase (kb) mRNA, whereas the rat liver transcript contains similar to 8 kb. In the present study, the primary structure of the enzyme encoded by the mouse 8-kb transcript is described. It is demonstrated that both the 4-and 8-kb transcripts have a wide tissue distribution and that they are encoded by separate genes. Characterization of the gene encoding the 4-kb transcript demonstrates that it spans a region of about 8 kb and that it contains at least 14 exons. The similarity of this gene and the previously characterized human gene for the 8-kb transcript is discussed.