A study of the viral etiology of histiocytic necrotizing lymphadenitis (Kikuchi-Fujimoto disease).
A study of the viral etiology of histiocytic necrotizing lymphadenitis (Kikuchi-Fujimoto disease).
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DOI：
10.3346/jkms.1998.13.1.27
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发表时间：
1998-02
影响因子：
4.5
通讯作者：
Gong G
中科院分区：
文献类型：
--
作者：
Huh J;Chi HS;Kim SS;Gong G
组织细胞坏死性淋巴结炎（HNL）或菊池病是一种独特的，自限性疾病的特点是坏死性颈部淋巴结病在年轻人。HNL在亚洲人中更为普遍，是韩国人中相对常见的疾病。先前的发热、淋巴细胞减少和偶尔的皮疹提示病毒病因，并且有零星的病毒相关报告。然而，到目前为止，还没有任何感染因子被证明与病原学有关。在本研究中，作者检查了HNL组织样本中疱疹病毒科基因组的存在。使用在EB病毒（EBV）DNA聚合酶基因的高度保守区域内选择的单对共有引物对12个新鲜冷冻的HNL淋巴结进行聚合酶链反应，旨在检测单纯疱疹病毒1型（HSV 1）、单纯疱疹病毒2型（HSV 2）和巨细胞病毒（CMV）以及EBV。然后通过单一限制性酶处理分析已知大小的扩增产物以进行确认。12例组织细胞坏死性淋巴结炎均未扩增出病毒DNA。作者得出结论，没有证据表明HSV 1、HSV 2、CMV或EBV在组织细胞坏死性淋巴结炎的发病机制中起任何作用。
Histiocytic necrotizing lymphadenitis (HNL) or Kikuchi's disease is a distinctive, self-limited disorder characterized by necrotizing cervical lymphadenopathy in young individuals. HNL is more prevalent among Asians and is a relatively common disorder among Koreans. A preceding fever, lymphopenia, and occasional skin rashes suggest a viral etiology and there have been sporadic reports of viral association. However, so far, no infectious agent has been proven to be etiologically related. In the present study, the authors examined HNL tissue samples for the presence of the genome of herpesviridae. A polymerase chain reaction was performed on 12 freshly frozen lymph nodes with HNL with a single pair of consensus primers selected within a highly conserved region of the DNA polymerase gene of the Epstein-Barr virus (EBV), designed to detect herpes simplex type 1 (HSV1), herpes simplex virus type 2 (HSV2), and cytomegalovirus (CMV) in addition to EBV. The amplified products of known sizes were then analyzed by a single restriction enzyme treatment for confirmation. No viral DNA was amplified in any of the 12 cases of histiocytic necrotizing lymphadenitis. The authors conclude that there is no evidence that HSV1, HSV2, CMV, or EBV plays any role in the pathogenesis of histiocytic necrotizing lymphadenitis.