Effects of chestnut tannins on intestinal morphology, barrier function, pro-inflammatory cytokine expression, microflora and antioxidant capacity in heat-stressed broilers
Effects of chestnut tannins on intestinal morphology, barrier function, pro-inflammatory cytokine expression, microflora and antioxidant capacity in heat-stressed broilers
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DOI：
10.1111/jpn.12839
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发表时间：
2018-06-01
影响因子：
2.7
通讯作者：
Deng, W.
中科院分区：
文献类型：
--
作者：
Liu, H. W.;Li, K.;Deng, W.
本试验旨在研究板栗单宁（CT）对热应激肉鸡肠道形态、屏障功能、促炎细胞因子表达、肠道菌群和抗氧化能力的影响。试验选用28日龄罗斯308肉鸡400只，随机分为4组，每组10个重复，每个重复10只鸡。正常（NOR）组温度为22 +/- 1℃，饲喂基础饲粮，其余3组分别为33 +/- 1℃（0800 ~ 1800℃）和22 +/- 1℃（1800 ~ 0800℃）循环加热，在基础饲粮中添加0 （HT）、1 （CT1）或2 (CT2) g CT/kg饲粮。试验期14 d。与HT组相比，NOR组和CT2组肉鸡平均日增重和空肠绒毛高度均高于对照组（p < 0.05），血清d-乳酸（p < 0.001）和二胺氧化酶（p < 0.01）水平低于对照组（p < 0.01）。饲粮中添加2 g CT/kg提高了热应激肉鸡空肠黏膜总抗氧化能力（p < 0.001）、超氧化物歧化酶活性（p < 0.05）和封闭带-1 mRNA表达水平（p < 0.05），降低了丙二醛浓度（p < 0.01）和白细胞介素-6 （p < 0.001）、核因子κ B (p < 0.001) mRNA表达水平。HT组肉鸡空肠（p < 0.01）和盲肠（p < 0.05）大肠杆菌和梭菌数量均高于NOR和CT2组。综上所述，饲粮中添加2 g CT/kg是缓解热应激对肉仔鸡生长性能和肠道功能不利影响的可行方法。
A study was conducted to evaluate the effects of chestnut tannins (CT) on intestinal morphology, barrier function, pro-inflammatory cytokine expression, microflora and antioxidant capacity in heat-stressed broilers. Four hundred 28-day-old male Ross 308 broilers were randomly assigned into four groups, with 10 replicates per group and 10 broilers per replicate. The broilers in the normal (NOR) group were kept at 22 +/- 1 degrees C and fed the basal diet, and each of the other three groups were treated with cyclic heat (33 +/- 1 degrees C from 0800 to 1800 and 22 +/- 1 degrees C from 1800 to 0800) and fed the basal diet with 0 (HT), 1 (CT1) or 2 (CT2) g of CT/kg of diet. The experiment lasted for 14 days. Compared with the HT group, broilers in the NOR and CT2 groups had higher (p < .05) average daily gain and villus height in the jejunum and lower serum d-lactate (p < .001) and diamine oxidase (p < .01) levels. The addition of 2 g CT/kg of diet increased the total antioxidant capacity (p < .001) and superoxide dismutase activities (p < .05) and zonula occludens-1 mRNA expression level (p < .05) and decreased the malondialdehyde concentration (p < .01) and mRNA expression levels of interleukin-6 (p < .001) and nuclear factor kappa B (p < .001) in the jejunal mucosa of heat-stressed broilers. The populations of Escherichia coli and Clostridium in the jejunum (p < .01) and caecum (p < .05) of broilers in the HT group were higher than those in the NOR and CT2 groups. In conclusion, the addition of 2 g CT/kg of diet seemed to be a feasible means of alleviating the negative effects of heat stress on the growth performance and intestinal function of broilers.