Early estrogen-induced nuclear changes in rat hypothalamic ventromedial neurons: an ultrastructural and morphometric analysis.
Early estrogen-induced nuclear changes in rat hypothalamic ventromedial neurons: an ultrastructural and morphometric analysis.
复制标题
大鼠下丘脑腹内侧神经元早期雌激素诱导的核变化：超微结构和形态测量分析。
DOI：
10.1002/cne.902390302
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
McEwen,BS
中科院分区：
文献类型：
--
作者：
Jones,KJ;Pfaff,DW;McEwen,BS
卵巢切除（OVX）大鼠暴露于雌二醇（E2）2小时或不连续暴露（E2 2小时/E2小时/E2小时/E2小时）后，腹内侧下丘脑神经元核的精细结构发生变化，先前表明这对于雌性大鼠的性行为是足够的（Parsons等人，’82 a），用电子显微镜检查。在光学显微镜水平上完成核仁、核和体面积以及核形状和周长的形态测量。Ez处理2小时后，核质的外观发生改变，异染色质的小而分散的团块减少。核的形状出现了显着的改变，从非球形，内陷的轮廓向球形，光滑的轮廓。核仁、核和体细胞肥大明显。此外，堆积的粗面内质网更频繁地出现在E2处理比对照OVX神经元。在不连续的（2小时、7小时、12小时）E2处理后，沿着核膜沿着以及核质中的小块异染色质逐渐丢失。沿核膜沿着的大异染色质团的减少与核仁相关染色质的增加相关。正如分布分析所确定的，这些雌激素诱导的细胞核变化共同发生的频率比预测的相互independence.These结果，显着的共同发生的E2诱导的变化，在30%或更多的细胞，和2小时Ez和2小时/7小时12小时组之间的差异是一致的级联细胞核变化后的头几个小时雌激素发作。
Alterations in the fine structure of nuclei of ventromedial hypothalamic neurons in ovariectomized (OVX) rats after either a 2-hour exposure to estradiol (E2) or a discontinuous exposure (2 hours E2/7 hours off12 hours Ez), previously shown sufficient for female rat sexual behavior (Parsons et al.,'82a), were examined with the electron microscope. Morphometric measurements of nucleolar, nuclear, and somal areas, and nuclear shape and perimeter were accomplished at the light microscope level. After 2 hours of Ez, the appearance of the nucleoplasm was altered, with a decrease in the small, scattered clumps of heterochromatin. Nuclear shape appeared dramatically altered from nonspherical, invaginated profiles toward spherical, smooth profiles. Nucleolar, nuclear, and somal hypertrophy were evident. In addition, stacked rough endoplasmic reticulum was present more frequently in E2-treated than control OVX neurons. After the discontinuous (2 hoursJ7 hours12 hours) E2 treatment, progressive loss of small clumps of heterochromatin along the nuclear envelope as well as in the nucleoplasm had occurred. Decrease in a large heterochromatin clump along the nuclear envelope was correlated with an increase in nucleolusassociated chromatin. As determined by a distribution analysis, these estrogen-induced nuclear changes co-occurred more frequently than predicted from mutual independence.These findings, the marked co-occurrence of E2-induced changes in 30% or more of the cells, and the differences between the 2-hour Ez and the 2-hour/7-hourl2-hour group are consistent with a cascade of cell nuclear changes in the first few hours after estrogen onset.