Activity-Based Therapies To Promote Forelimb Use after a Cervical Spinal Cord Injury
Activity-Based Therapies To Promote Forelimb Use after a Cervical Spinal Cord Injury
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DOI：
10.1089/neu.2008.0592
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发表时间：
2009-10-01
影响因子：
4.2
通讯作者：
Bregman, Barbara S.
中科院分区：
文献类型：
--
作者：
Dai, Haining;MacArthur, Linda;Bregman, Barbara S.
人们对改善脊髓损伤后前肢功能的策略非常感兴趣。我们研究了丰富的住房与熟练训练相结合对成年大鼠颈髓损伤后熟练和自动前肢功能恢复的影响。所有动物都接受了熟练的伸展、穿越网格路和地上运动的预训练。一些人在 C4-5 处接受了颈椎过度半切损伤，中断了右侧脊髓和双侧背柱，并被单独安置在标准住房中或进行日常训练的丰富环境中。一部分动物接受咯利普兰以促进神经元可塑性。连续 4 周，每周对动物进行一次测试，以测量动物的伸展、网格行走误差、运动和垂直探索。将生物素化的葡聚糖胺注射到皮质中以标记皮质脊髓束。与标准住房相比，丰富的环境/日常训练显着增加了左前伸的数量和成功率。动物在网格行走（衡量协调前肢功能的指标）上犯的错误也更少。然而，在垂直探索或运动过程中前肢的使用没有显着改善。同样，咯利普兰没有改善任何测试的行为。富集外壳和咯利普兰都增加了病变头侧皮质脊髓束的可塑性。这些研究表明，颈髓损伤后的熟练训练可以改善前肢熟练使用（伸展）和协调肢体功能（网格行走）的恢复，但不会改善自动前肢功能（运动和垂直探索）。这些研究表明，脊髓损伤后恢复前肢功能需要针对下行通路和节段通路采取单独的策略。
Significant interest exists in strategies for improving forelimb function following spinal cord injury. We investigated the effect of enriched housing combined with skilled training on the recovery of skilled and automatic forelimb function after a cervical spinal cord injury in adult rats. All animals were pretrained in skilled reaching, gridwalk crossing, and overground locomotion. Some received a cervical over-hemisection lesion at C4-5, interrupting the right side of the spinal cord and dorsal columns bilaterally, and were housed in standard housing alone or enriched environments with daily training. A subset of animals received rolipram to promote neuronal plasticity. Animals were tested weekly for 4 weeks to measure reaching, errors on the gridwalk, locomotion, and vertical exploration. Biotinylated dextran amine was injected into the cortex to label the corticospinal tract. Enriched environments/daily training significantly increased the number and success of left reaches compared to standard housing. Animals also made fewer errors on the gridwalk, a measure of coordinated forelimb function. However, there were no significant improvements in forelimb use during vertical exploration or locomotion. Likewise, rolipram did not improve any of the behaviors tested. Both enriched housing and rolipram increased plasticity of the corticospinal tract rostral to the lesion. These studies indicate that skilled training after a cervical spinal cord injury improves recovery of skilled forelimb use (reaching) and coordinated limb function (gridwalk) but does not improve automatic forelimb function (locomotion and vertical exploration). These studies suggest that rehabilitating forelimb function after spinal cord injury will require separate strategies for descending and segmental pathways.