Ecologically differentiated stress-tolerant endosymbionts in the dinoflagellate genus Symbiodinium (Dinophyceae) Clade D are different species
Ecologically differentiated stress-tolerant endosymbionts in the dinoflagellate genus Symbiodinium (Dinophyceae) Clade D are different species
复制标题
DOI：
10.2216/13-186.1
复制
发表时间：
2014-01-01
期刊：
影响因子：
1.6
通讯作者：
Chen, Chaolun Allen
中科院分区：
文献类型：
--
作者：
LaJeunesse, Todd C.;Wham, Drew C.;Chen, Chaolun Allen
我们使用了一种整合遗传学方法，使用（1）核核糖体rDNA序列（内部转录间隔区和部分大亚基rDNA），（2）单拷贝微卫星核DNA，（3）叶绿体编码的23 S rDNA，（4）线粒体细胞色素B，和（5）8个微卫星标记的重复变异，以检验耐胁迫，共生甲藻（Dinophyceae）D分支由一个以上的种组成。一致的系统发育和种群遗传证据清楚地区分单独发展，生殖隔离谱系。本文描述了北方共生甲藻（Symbiodiniumboreum sp. nov.）广体藻属新种，已知存在于斑马珊瑚群落中的两种共生体，即皱珊瑚Oulastrea crispata（Scleractinia），它生活在从赤道东南亚延伸到温带西北太平洋日本主要岛屿的浑浊边缘栖息地。共生甲藻与O. crispata和S.广速幼体在热带的群体中很常见。这两种共生体的地理范围重叠，在亚热带，他们有时共同出现在同一个主机殖民地。本文还描述了川氏共生甲藻。作为一种宿主-通才共生体，它经常与各种具有开放（水平）共生体获取模式的石珊瑚共生，并且在西太平洋、印度洋、阿拉伯湾/波斯湾、红海和西大西洋（加勒比海）的温暖浑浊的珊瑚礁栖息地中繁荣的珊瑚礁群落中很常见。作为典型的甲藻，S。boreum和S. eurythalpos是单倍体，而来自田间采集和培养的S. trenchii通常具有两个等位基因，这意味着在该物种的进化过程中发生了全基因组复制。认识到分支D共生藻包含的物种在宿主特异性和地理分布上表现出显着的差异，将产生更大的科学清晰度如何耐应力共生体功能的珊瑚甲藻共生生态响应全球气候变化。
We used an integrative genetics approach using sequences of (1) nuclear ribosomal rDNA (internal transcribed spacers and partial large subunit rDNA), (2) single-copy microsatellite nuclear DNA, (3) chloroplast-encoded 23S rDNA, (4) mitochondrial cytochrome b, and (5) repeat variation at eight microsatellite markers, to test the hypothesis that the stress-tolerant, 'morphologically cryptic' Clade D Symbiodinium (Dinophyceae) was composed of more than one species. Concordant phylogenetic and population genetic evidence clearly differentiate separately evolving, reproductively isolated lineages. We describe Symbiodinium boreum sp. nov. and S. eurythalpos sp. nov., two symbionts known to occur in colonies of the zebra coral, Oulastrea crispata (Scleractinia), which lives in turbid, marginal habitats extending from equatorial Southeast Asia to the main islands of Japan in the temperate northwest Pacific Ocean. Symbiodinium boreum was associated with O. crispata in temperate latitudes and S. eurythalpos was common to colonies in the tropics. The geographical ranges of both symbiont species overlapped in the subtropics where they sometimes co-occurred in the same host colony. Symbiodinium trenchii sp. nov. is also described. As a host-generalist symbiont, it often occurs in symbiosis with various species of Scleractinia possessing open (horizontal) modes of symbiont acquisition and is common to reef coral communities thriving in warm turbid reef habitats in the western Pacific Ocean, Indian Ocean, Arabian/Persian Gulf, Red Sea and western Atlantic (Caribbean). As is typical for dinoflagellates, S. boreum and S. eurythalpos were haploid, but microsatellite loci from field-collected and cultured S. trenchii often possessed two alleles, implying that a genome-wide duplication occurred during the evolution of this species. The recognition that Clade D Symbiodinium contains species exhibiting marked differences in host specificity and geographical distribution will yield greater scientific clarity about how stress-tolerant symbionts function in the ecological response of coral-dinoflagellate symbioses to global climate change.