Trichomonas adhere and phagocytose sperm cells:: adhesion seems to be a prominent stage during interaction
Trichomonas adhere and phagocytose sperm cells:: adhesion seems to be a prominent stage during interaction
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DOI：
10.1007/s00436-007-0793-3
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发表时间：
2008-03-01
影响因子：
2
通讯作者：
Burla Dias, Angelo Jose
中科院分区：
文献类型：
--
作者：
Benchimol, Marlene;Rosa, Ivone de Andrade;Burla Dias, Angelo Jose
胎儿毛滴虫和阴道毛滴虫分别是牛和人泌尿生殖道的细胞外寄生虫。它们会导致不孕和流产，但没有关于牛精子细胞对这种牛寄生虫的敏感性的记录信息。这项工作的目的是研究胎儿毛滴虫和阴道毛滴虫与牛和人类精子细胞相互作用时引起的影响。牛和人的精子分别取自未感染的公牛和男性，并在不同时期暴露于活滴虫。光学显微镜、视频显微镜、扫描和透射电子显微镜首先揭示了这两种不同细胞之间的向性，然后是紧密接近，然后是紧密粘附。观察到精子活力下降以及精液凝集强烈。毛滴虫与精子细胞之间的粘附是通过鞭毛或精子头发生的。在接下来的 12 小时内观察到能活动的寄生虫，而与寄生虫接触的精子细胞很快就变得不能活动。寄生虫能够维持精子细胞附着在其细胞表面，然后进行吞噬作用。这个过程始于紧密的膜-膜粘附以及精子细胞并入细胞内液泡。随后，精子细胞在溶酶体中逐渐被消化。许多滴虫在与精子接触时受伤和/或死亡，可能是由于坏死。这项研究的结果表明，胎儿毛滴虫和阴道毛滴虫都与精子细胞相互作用，引起这些生殖细胞的损伤和死亡。两种毛滴虫的行为差异很明显，表明阴道毛滴虫比胎儿毛滴虫的毒性要强得多。讨论了滴虫在生殖失败中的可能作用。
Tritrichomonas foetus and Trichomonas vaginalis are extracellular parasites of the urogenital tract of cattle and humans, respectively. They cause infertility and abortion, but there is no documented information on the susceptibility of bovine sperm cells to this cattle parasite. The aim of this present work was to study the effects provoked by T. foetus and T. vaginalis when in interaction with bovine and human sperm cells. The bovine and human spermatozoa were obtained from uninfected bulls and men, respectively, and were exposed to living trichomonads over different periods of time. Light microscopy, video microscopy, scanning, and transmission electron microscopy first revealed a tropism, then a close proximity followed by a tight adhesion between these two different cells. A decrease in the spermatozoa motility was observed as well intense semen agglutination. The adhesion between trichomonads to the sperm cell occurred either by the flagella or sperm head. Motile parasites were observed during the next 12 h, whereas sperm cells in contact with the parasites rapidly became immotile. The parasites were able to maintain the sperm cells attached to their cell surface, followed by phagocytosis. This process began with a tight membrane-membrane adhesion and the incorporation of the sperm cell within an intracellular vacuole. Afterwards, the sperm cell was gradually digested in lysosomes. Many trichomonads were injured and/or died on making contact with the spermatozoa possibly due to necrosis. Results from this study demonstrated that both T. foetus and T. vaginalis interact with sperm cells provoking damage and death of these reproductive cells. Differences in the behavior of both trichomonads were evident, showing that T. vaginalis was much more virulent than T. foetus. The possible role of trichomonads in reproductive failure is discussed.