Enhancement of Electricity Production of Microbial Fuel Cells by Using DNA Nanostructures as Electron Mediator Carriers
Enhancement of Electricity Production of Microbial Fuel Cells by Using DNA Nanostructures as Electron Mediator Carriers
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DOI：
10.1021/acssuschemeng.2c04399
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发表时间：
2022-11-22
影响因子：
8.4
通讯作者：
Wang, Risheng
中科院分区：
文献类型：
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作者：
Han, Shuo;Thapa, Krishna;Wang, Risheng
微生物燃料电池（Microbial fuel cells，MFC）是一种以生物质和微生物为燃料，将废弃物中的化学能转化为电能的生态友好型技术。在这里，一个三维的DNA折纸纳米结构作为电子介体-亚甲基蓝（MB）载体，首次被用来提高电子的生产和转移在阳极室的大肠杆菌系统为基础的MFC。通过将MB分子负载在DNA折纸纳米结构上，与裸CF和MB修饰的CF电极相比，具有MB/DNA折纸修饰的碳毡（CF）电极的MFC显示出最高的电压产生（64 mV）和功率密度（5.78 mW/m2）。MFC性能的提高归因于DNA折纸辅助MB负载的较大界面面积和阳极上的生物相容性细菌生长环境，这导致了E.大肠杆菌粘附和快速电子传递。此外，具有MB/DNA折纸修饰的MFC可以在恒定电压放电下稳定运行三个循环（20天）而无需进一步添加培养基。这些结果表明，DNA折纸是一种很有前途的材料，可以作为可持续能源系统的电子介体载体，可以克服无载体MFC的缺点，例如寿命短、不断添加补给以及对微生物和自然环境的毒性。
Microbial fuel cells (MFCs) are recognized as eco-friendly technology to convert chemical energy from waste into electricity by biocatalytic microorganisms and biomass as fuel feedstocks. Here, a three-dimensional DNA origami nanostructure serving as electron mediator-methylene blue (MB) carriers was first employed to enhance the electron production and transfer in the anode compartment of Escherichia coli system-based MFCs. By loading MB molecules on DNA origami nanostructures, the MFC with the MB/DNA origami-modified carbon felt (CF) electrode showed the highest voltage production (64 mV) and power density (5.78 mW/m2) compared to bare CF and MB-modified CF electrodes. The enhanced MFC performance was attributed to the larger interface area of DNA origami-assisted MB loading and a biocompatible bacterial growth environment on the anode, which led to E. coli adhesion and fast electron transfer. Furthermore, the MFC with MB/DNA origami modifications could stably operate for three cycles (20 days) with constant voltage discharge without further addition of media. These results show that DNA origami is a promising material serving as an electron mediator carrier for sustainable energy systems, which could get over the drawbacks of carrier-free MFCs, such as short lifetime, continuously adding supplies, and toxicity to both the microorganisms and the natural environment.