Simultaneous multispectral reflectance imaging and laser speckle flowmetry of cerebral blood flow and oxygen metabolism in focal cerebral ischemia
Simultaneous multispectral reflectance imaging and laser speckle flowmetry of cerebral blood flow and oxygen metabolism in focal cerebral ischemia
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DOI：
10.1117/1.2950312
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发表时间：
2008-07-01
影响因子：
3.5
通讯作者：
Dunn, Andrew K.
中科院分区：
文献类型：
--
作者：
Jones, Phill B.;Shin, Hwa Kyoung;Dunn, Andrew K.
由于激光多普勒血流仪和磁共振成像（MRI）等技术的空间和时间分辨率有限，直到最近，脑血流量（CBF）和氧合血红蛋白浓度（HbO，HbR）动态的实时调查一直很困难。激光散斑血流仪（LSF）和多光谱反射成像（MSRI）的组合产生响应于功能性皮层激活的血流动力学和代谢变化的高分辨率时空图。在急性局灶性脑缺血期间，HbO和HbR的变化远大于功能激活的变化，导致Beer-Lambert近似法无法得出准确的结果。我们描述了同时使用LSF和MSRI，使用非线性Monte Carlo拟合技术，记录远端大脑中动脉闭塞（dMCAO）诱导的急性局灶性脑缺血和再灌注期间CBF、HbO、HbR和脑氧代谢率（CMRO（2））的快速变化。该技术通过完整颅骨以高时间和空间分辨率捕获血液动力学和代谢事件期间的CBF和CMRO（2）变化，并证明了同时LSF和MSRI在脑血管疾病小鼠模型中的实用性。(C)2008年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
Real-time investigation of cerebral blood flow (CBF), and oxy-and deoxyhemoglobin concentration (HbO, HbR) dynamics has been difficult until recently due to limited spatial and temporal resolution of techniques like laser Doppler flowmetry and magnetic resonance imaging (MRI). The combination of laser speckle flowmetry (LSF) and multispectral reflectance imaging (MSRI) yields high-resolution spatiotemporal maps of hemodynamic and metabolic changes in response to functional cortical activation. During acute focal cerebral ischemia, changes in HbO and HbR are much larger than in functional activation, resulting in the failure of the Beer-Lambert approximation to yield accurate results. We describe the use of simultaneous LSF and MSRI, using a nonlinear Monte Carlo fitting technique, to record rapid changes in CBF, HbO, HbR, and cerebral metabolic rate of oxygen (CMRO(2)) during acute focal cerebral ischemia induced by distal middle cerebral artery occlusion (dMCAO) and reperfusion. This technique captures CBF and CMRO(2) changes during hemodynamic and metabolic events with high temporal and spatial resolution through the intact skull and demonstrates the utility of simultaneous LSF and MSRI in mouse models of cerebrovascular disease. (C) 2008 Society of Photo-Optical Instrumentation Engineers.