Ovarian cancer cell lines derived from non-serous carcinomas migrate and invade more aggressively than those derived from high-grade serous carcinomas
Ovarian cancer cell lines derived from non-serous carcinomas migrate and invade more aggressively than those derived from high-grade serous carcinomas
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DOI：
10.1038/s41598-019-41941-4
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发表时间：
2019-04-02
影响因子：
4.6
通讯作者：
Herrington, C. Simon
中科院分区：
文献类型：
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作者：
Hallas-Potts, Amelia;Dawson, John C.;Herrington, C. Simon
术语卵巢癌描述了在卵巢中生长但不一定是卵巢起源的一组异质性肿瘤。最近的基因组分析表明，许多最常用的卵巢癌细胞系的特征被错误描述，导致错误的结论，并在将实验室研究转化为患者的新型治疗方法方面存在差距。在这里，我们使用10个上皮性卵巢癌细胞系来研究2D迁移，细胞周期参数和3D侵入行为到不同的基板，并发现来自不同来源的细胞系的行为之间的显着差异。来自非浆液性癌的细胞系迁移更快，更容易侵入基质胶和胶原蛋白I基板比来自高级别浆液性癌的细胞系。然而，并非所有来源于非浆液性癌的细胞系都表现出类似的侵袭行为。这些发现可能反映了细胞系来源的原发性肿瘤类型的行为差异，因为高级别浆液性癌通常在腹膜表面扩张和扩散。这些结果为鉴定卵巢癌肿瘤类型之间的差异提供了体外模型的基础。
The term ovarian cancer describes a heterogeneous group of tumours that grow in the ovary but are not necessarily of ovarian origin. Recent genomic analysis has shown that many of the most commonly used ovarian cancer cell lines have been mischaracterised, leading to erroneous conclusions and a gap in the translation of laboratory research into novel treatments for patients. Here, we use 10 epithelial ovarian cancer cell lines to investigate 2D migration, cell cycle parameters and 3D invasion behaviour into different substrates and find significant differences between the behaviours of cell lines from different origins. Cell lines derived from non-serous carcinomas migrated more quickly and were more likely to invade into Matrigel and collagen I substrates than cell lines derived from high-grade serous carcinomas. However not all cell lines derived from non-serous carcinomas exhibited similar invasive behaviour. These findings may reflect differences in the behaviour of the primary tumour types from which the cell lines were derived, given that high-grade serous carcinomas typically expand and spread over peritoneal surfaces. These results provide the basis of an in vitro model for identifying differences between ovarian cancer tumour types.