Proteomic analysis of membrane proteins from a radioresistant and moderate thermophilic bacterium Deinococcus geothermalis
Proteomic analysis of membrane proteins from a radioresistant and moderate thermophilic bacterium Deinococcus geothermalis
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对耐辐射和中度嗜热地热奇球菌的膜蛋白进行蛋白质组学分析
DOI：
10.1039/c004875e
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Hua, Yuejin
中科院分区：
文献类型：
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作者：
Tian, Bing;Wang, Hu;Hua, Yuejin
地热球菌是一种耐辐射中等嗜热性细菌。对这种细菌的膜或膜相关蛋白知之甚少。本研究以Triton X-114洗涤剂提取液为模板，采用一维PAGE和LC-MS/MS分析方法，建立了地温假单胞菌的膜亚蛋白质组图谱。从两个独立的试验中鉴定了来自膜制剂的总共552种蛋白质。在鉴定的蛋白质中，117个为膜亚蛋白质组蛋白，其中89个为首次报道，包括菌毛蛋白(DGEO_2038)、细胞色素BD泛喹酚氧化酶(DGEO_2705)和多传感器(DGEO_2096)。主要的膜亚蛋白质组蛋白分为18个功能基团，包括营养物质的运输和代谢、能量的产生和转化、细胞壁/膜的生物发生和一个功能较差的亚类。通过对细胞表面受体IPT/TIG(DGEO_1119)和4种假想蛋白的鉴定，证实了该菌细胞膜上具有特殊的蛋白质组成和功能。这些结果为定量蛋白质组学分析提供了基础，这将有助于了解该生物体对不同环境胁迫的适应情况，并制定环境废物的生物修复策略。
Deinococcus geothermalis is a radioresistant and moderate thermophilic bacterium. Little was known about the membrane or membrane associated proteins of this bacterium. This study established the membrane subproteome profile of D. geothermalis, using 1-D PAGE and LC-MS/MS analysis following Triton X-114 detergent extraction. A total of 552 proteins from the membrane preparations were identified from two independent trials. In the total identified proteins, 117 were membrane subproteomic proteins, and 89 of them were described for the first time in D. geothermalis including fimbrial pilin (Dgeo_2038), cytochrome bd ubiquinol oxidase (Dgeo_2705) and multi-sensor (Dgeo_2096). The major membrane subproteomic proteins were distributed into 18 functional groups including nutrient transport and metabolism, energy production and conversion, cell wall/membrane biogenesis and a poorly characterized subclass. The identifications of Deinococcus-specific proteins, such as cell surface receptor IPT/TIG (Dgeo_1119) and four hypothetical proteins, demonstrated the special protein composition and functions in the cell membrane of Deinococcus. The results provide a basis for quantitative proteomic analysis, which will facilitate the understanding of the adaptation of this organism to different environmental stresses and the development of strategies for bioremediation of environmental waste.