E2F1 and E1A(12S) have a homologous activation domain regulated by RB and CBP.
E2F1 and E1A(12S) have a homologous activation domain regulated by RB and CBP.
复制标题
E2F1和E1A(12S)具有受RB和CBP调节的同源激活结构域。
DOI：
--
复制
发表时间：
1996
影响因子：
11.1
通讯作者：
T. Kouzarides
中科院分区：
文献类型：
--
作者：
D. Trouche;T. Kouzarides
The E2F1 transcription factor has a well-characterized activation domain at its C terminus and the E1A protein has a recently defined activation domain at its N terminus. Here we show that these activation domains are highly related in sequence. The sequence homology reflects, at least partly, the conservation of common binding sites for the RB and CBP/p300 proteins, which are preserved in the same relative order along E2F1 and E1A. Furthermore, the interaction of RB and CBP with these two activation domains results in the same functional consequences: RB represses both activation domains, whereas CBP stimulates them. We conclude that the activation domains of E1A(12s) and E2F1 belong to a novel functional class, characterized by specific protein binding sites. The implication of this conservation with respect to E1A-induced stimulation of E2F activity is discussed.
