Sensitive electrochemical detection of cardiac troponin I in serum and saliva by nitrogen-doped porous reduced graphene oxide electrode
Sensitive electrochemical detection of cardiac troponin I in serum and saliva by nitrogen-doped porous reduced graphene oxide electrode
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DOI：
10.1016/j.snb.2018.01.215
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发表时间：
2018-06-01
影响因子：
8.4
通讯作者：
Szunerits, Sabine
中科院分区：
文献类型：
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作者：
Chekin, Fereshteh;Vasilescu, Alina;Szunerits, Sabine
心血管疾病是发达国家所有疾病中死亡风险最高的疾病之一，不断增加卫生系统的负担。因此，心血管疾病的早期诊断对于降低死亡率和采用更适应的治疗决策变得非常重要。我们在这里证明了氮掺杂的还原氧化石墨烯（N-prGO）在生理相关条件下用于检测和定量心肌肌钙蛋白I（cTnI）（一种关键的人类心脏蛋白生物标志物）的实用性。通过1-芘羧酸（py-COOH）和聚（乙二醇）修饰的芘（py-PEG）配体对N-prGO的非共价修饰允许以其对cTnI的高选择性而已知的Tro 4适体的共价整合。利用微分脉冲伏安法（DPV），可获得一种检测人血清中浓度低至1 pgmL（-1）的cTnI的免标记电化学传感器。这种灵敏的检测源于具有优异电化学性能的多孔纳米材料的集成，该多孔纳米材料易于进行特定位点的表面改性。(C)2018爱思唯尔B. V.保留所有权利。
Cardiovascular diseases pose one of the highest mortality risks among all diseases in developed countries, steadily increasing the burden on the health systems. Early diagnosis of cardiovascular diseases has consequently become highly important to decrease mortality and to use more adapted therapeutic decisions. We demonstrate here the utility of nitrogen-doped reduced graphene oxide (N-prGO) for detecting and quantifying of cardiac troponin I (cTnI), a key human cardiac protein biomarker, under physiologically relevant conditions. Non-covalent modification of N-prGO by 1-pyrenecarboxylic acid (py-COOH) and poly(ethylene glycol) modified pyrene (py-PEG) ligands allowed the covalent integration of Tro4 aptamer, known for its high selectivity towards cTnI. Using differential pulse voltammetry (DPV), a label-free electrochemical sensor for cTnI for concentrations down to 1 pgmL(-1) in human serum could be obtained. This sensitive detection arises from the integration of a porous nanomaterial with excellent electrochemical properties being easily amendable to site-specific surface modification. (C) 2018 Elsevier B.V. All rights reserved.