Improved stability, epithelial permeability and cellular antioxidant activity of β-carotene via encapsulation by self-assembled α-lactalbumin micelles
Improved stability, epithelial permeability and cellular antioxidant activity of β-carotene via encapsulation by self-assembled α-lactalbumin micelles
复制标题
通过自组装 α-乳清蛋白胶束封装，提高 β-胡萝卜素的稳定性、上皮通透性和细胞抗氧化活性
DOI：
10.1016/j.foodchem.2018.07.216
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发表时间：
2019-01-15
期刊：
影响因子：
8.8
通讯作者：
Li, Yuan
中科院分区：
文献类型：
--
作者：
Du, Yizheng;Bao, Cheng;Li, Yuan
疏水性生物活性化合物如β-胡萝卜素（beta-carotene，beta-c）的水溶性、稳定性和生物利用度低，极大地阻碍了其在食品中的应用。纳米载体可以通过促进功能成分的递送来克服这个问题。我们在水溶液中通过部分酶解制备了乳白蛋白（α-lac）胶束。β-C可以通过疏水相互作用结合到这些胶束的核心中。与游离β-c相比，包封的β-c在60 ℃加热或UV光照射下的水溶性和稳定性显著改善。此外，在Caco-2细胞单层模型中，它具有增加的细胞摄取（3倍）和跨膜渗透性（13倍）。表明α-紫胶胶束包裹的β-c具有增强的细胞吸收和转运效率。与游离β-c相比，包封的β-c也表现出增强的细胞抗氧化活性（CAA）。这项工作表明，α-紫胶胶束显示出巨大的潜力，在食品中的疏水性生物活性化合物的交付。
The low aqueous solubility, stability and bioavailability of hydrophobic bioactive compounds, such as beta-carotene (beta-c), greatly hinder their application in foods. Nanocarriers could overcome this problem by facilitating the delivery of the functional ingredients. We prepared lactalbumin (alpha-lac) micelles by partial enzymatic hydrolysis in aqueous solution. beta-c can be incorporated into the cores of these micelles via hydrophobic interactions. The aqueous solubility and stability under 60 degrees C heating or UV light irradiation of encapsulated beta-c improved significantly compared with free beta-c. Moreover, it had an increased cellular uptake (3 times) and transmembrane permeability (13 times) in a Caco-2 cell monolayer model. It suggested that alpha-lac micelle-encapsulated beta-c had an enhanced cellular absorption and transport efficiency. Encapsulated beta-c also exhibited an enhanced cellular anti-oxidant activity (CAA) compared with free beta-c. This work demonstrates that alpha-lac micelles showed a great potential for delivery of hydrophobic bioactive compounds in foods.