Expression of aldehyde dehydrogenase 6 reduces inhibitory effect of furan derivatives on cell growth and ethanol production in Saccharomyces cerevisiae.
Expression of aldehyde dehydrogenase 6 reduces inhibitory effect of furan derivatives on cell growth and ethanol production in Saccharomyces cerevisiae.
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DOI：
10.1016/j.biortech.2011.02.101
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发表时间：
2011-05
影响因子：
11.4
通讯作者：
Seongeon Park;H. Koo;Young-Kyoung Park;S. M. Park;J. C. Park;Oh-Kyu Lee;Yong-Cheol Park;Jin-Ho Seo
中科院分区：
文献类型：
--
作者：
Seongeon Park;H. Koo;Young-Kyoung Park;S. M. Park;J. C. Park;Oh-Kyu Lee;Yong-Cheol Park;Jin-Ho Seo
酵母脱氢酶和还原酶在酿酒酵母 D452-2 中过度表达，以解毒 2-糠醛（糠醛）和 5-羟甲基糠醛（HMF）（酸水解纤维素生物质中存在的两种有效有毒化学物质），从而改善细胞生长和乙醇生产。在这些酶中，醛脱氢酶6（ALD6）发挥着直接氧化呋喃衍生物和为其还原反应提供NADPH辅因子的双重作用。与野生型菌株相比，在 2g/L 糠醛和 0.5g/L HMF 存在下，酿酒酵母 D452-2/pH-ALD6 的分批发酵导致比生长率、乙醇浓度和乙醇生产率提高 20-30%。有人提出，ALD6 的过度表达可以恢复酵母细胞代谢，从而增加含有呋喃衍生抑制剂的木质纤维素生物质的乙醇产量。
Yeast dehydrogenases and reductases were overexpressed in Saccharomyces cerevisiae D452-2 to detoxify 2-furaldehyde (furfural) and 5-hydroxymethyl furaldehyde (HMF), two potent toxic chemicals present in acid-hydrolyzed cellulosic biomass, and hence improve cell growth and ethanol production. Among those enzymes, aldehyde dehydrogenase 6 (ALD6) played the dual roles of direct oxidation of furan derivatives and supply of NADPH cofactor to their reduction reactions. Batch fermentation of S. cerevisiae D452-2/pH-ALD6 in the presence of 2g/L furfural and 0.5g/L HMF resulted in 20–30% increases in specific growth rate, ethanol concentration and ethanol productivity, compared with those of the wild type strain. It was proposed that overexpression of ALD6 could recover the yeast cell metabolism and hence increase ethanol production from lignocellulosic biomass containing furan-derived inhibitors.