Inactivation of bradykinin in the pulmonary circulation.
Inactivation of bradykinin in the pulmonary circulation.
复制标题
缓激肽在肺循环中的失活。
DOI：
10.1042/bj1100795
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发表时间：
1968
影响因子：
4.1
通讯作者：
J. Stewart
中科院分区：
文献类型：
--
作者：
J. Ryan;J. Roblero;J. Stewart
Ferreira&Vane(1967)已经证明，注入右心房的缓激肽只有不到30%出现在升主动脉。在血液中，发现生物半衰期为17秒。考虑到从右心房到升主动脉的短暂循环时间(2-Losec.；Spector，1956)，他们得出结论，血液中的酶不能解释肺循环中发生的失活程度。肺内缓激肽失活的一个可能机制是通过血管内皮细胞或血管内皮细胞附近的酶进行水解。我们已经开始了一项关于这种可能性的研究，使用了一种在原位洗去血的大鼠肺制剂。大鼠体重0-2-0-4公斤，用戊巴比妥钠(50 mg/kg)麻醉。车身重量。)通过腹腔注射给药。在左侧颈总动脉和右侧颈外静脉置入聚乙烯管。另一根插管被放置在气管内，肺部接受机械通气。将生理盐水(0.9%NaCI)注入静脉插管，并通过动脉插管放血。然后，将一根插管通过右心室的缝隙插入肺动脉，另一根导管捆绑在左心室。洛克葡聚糖溶液(11.含有：右旋糖苷40g；氯化钠90g；氯化钾0-42g；氯化钙0-24g；D-葡萄糖10g；碳酸氢钠0.2g。)以4ml./min的速度注入肺动脉。从左心室插管收集流出物。输液在370℃加热，充气02+CO2(95：5)。当流出物中没有红细胞时(3040分钟后)[2-L-脯氨酸-14C]缓激肽(28.6uc/mg，批号307-；新英格兰核公司，波士顿，马萨诸塞州，美国)在Locke中注入葡聚糖溶液。输液速度维持在4ml./min。缓激肽在2-0或4 0，ug。
Ferreira & Vane (1967) have shown that less than 30% of bradykinin infused into the right atrium emerges in the ascending aorta. In blood the biological half-life was found to be 17 sec. Considering the brief circulation time from right atrium to ascending aorta (2-lOsec.; Spector, 1956), they concluded that enzymes of blood could not account for the degree of inactivation that occurred in the pulmonary circulation. A possible mechanism for the pulmonary inactivation of bradykinin is through hydrolysis by enzymes at or near the vascular endothelium. We have begun an investigation of this possibility by using a rat lung preparation in situ washed free of blood. Rats, weighing 0-2-0-4kg., were anaesthetized with pentobarbital (50mg./kg. body wt.) given by intraperitoneal injection. Polythene cannulae were placed in the left common carotid artery and the right external jugular vein. Another cannula was placed in the trachea and the lungs were ventilated mechanically. Saline (0.9% NaCI) was infused into the venous cannula and the rat was exsanguinated through the arterial cannula. Then a cannula was inserted through a slit in the right ventricle into the pulmonary artery and another was tied into the left ventricle. A Locke dextran solution (11. contained: dextran, 40g.; NaCl, 9 0g.; KCl, 0-42g.; CaCl2, 0-24g.; D-glucose, 10g.; NaHCO3, 0.2g.) was infused into the pulmonary artery at a rate of 4ml./min. and the effluent was collected from the cannula in the left ventricle. The infusion solutions were heated at 370 and were aerated with 02 + CO2 (95: 5). When the effluent became free of erythrocytes (after 3040min.) [2-L-proline-14C]bradykinin (28.6 ,uc/mg., lot no. 307-64; New England Nuclear Corp., Boston, Mass., U.S.A.) in Locke dextran solution was infused. The infusion rate was maintained at 4ml./min. Bradykinin was infused at 2-0 or 4 0,ug./