Genetics of isoprenoid biosynthesis in Paracoccus zeaxanthinifaciens
Genetics of isoprenoid biosynthesis in Paracoccus zeaxanthinifaciens
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DOI：
10.1016/s0378-1119(02)00877-6
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发表时间：
2002-09-04
期刊：
影响因子：
3.5
通讯作者：
Berry, A
中科院分区：
文献类型：
--
作者：
Hümbelin, M;Thomas, A;Berry, A
对玉米黄质产生菌Paracoccus zeaxanthinifaciens中乙酰辅酶A转化为法呢基二磷酸（FPP）的所有酶的编码基因进行了克隆和表征。发现了两个编码催化两个乙酰辅酶A分子缩合为乙酰乙酰辅酶A的酶的基因，将参与乙酰辅酶A和乙酰乙酰辅酶A转化为异戊烯基二磷酸（IPP）和二甲基烯丙基二磷酸（DMAPP）的六个酶集中在一个操纵子中，命名为甲羟戊酸操纵子。编码催化两个连续缩合（IPP和DMAPP到香叶基二磷酸（GPP）以及IPP和GPP到FPP）的酶的基因不与编码类异戊二烯途径的酶的任何其它基因成簇。还提供了在玉米黄质假单胞菌中发现的编码参与聚羟基链烷酸酯和非类胡萝卜素类异戊二烯生物合成的酶的基因。(C)2002 Elsevier Science B.V.保留所有权利。
The genes coding for all the enzymes involved in the conversion of acetyl-CoA to farnesyl diphosphate (FPP) in the zeaxanthin-producing bacterium Paracoccus zeaxanthinifaciens were cloned and characterized. Two genes encoding enzymes catalysing the condensation of two acetyl-CoA molecules to acetoacetyl-CoA were found. The six enzymes involved in the conversion of acetyl-CoA and acetoacetyl-CoA to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) are grouped in an operon, designated the mevalonate operon. The gene encoding the enzyme catalysing two consecutive condensations, IPP and DMAPP to geranyl diphosphate (GPP) and IPP and GPP to FPP, is not clustered with any other gene encoding an enzyme of the isoprenoid pathway. Genes encoding enzymes involved in the biosynthesis of poly-hydroxyalkanoate and non-carotenoid isoprenoids found in P. zeaxanthinifaciens are also presented. (C) 2002 Elsevier Science B.V. All rights reserved.