Rab11 is an apically located small GTP-binding protein in epithelial tissues
Rab11 is an apically located small GTP-binding protein in epithelial tissues
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DOI：
10.1152/ajpgi.1996.270.3.g515
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发表时间：
1996-03-01
影响因子：
4.5
通讯作者：
Navarre, J
中科院分区：
文献类型：
--
作者：
Goldenring, JR;Smith, J;Navarre, J
Rab蛋白参与了真核细胞内动态囊泡加工的许多方面。我们先前已经在胃壁细胞、小管泡膜中发现了Rab11。我们已经研制出一种针对Rab11的单抗。在所检测的所有兔组织中，Rab11免疫反应在上皮细胞中高度丰富。在胃底，壁细胞的染色模式与管泡上的定位一致。胃底和胃窦的表面粘液细胞均呈点状根尖下染色。回肠和近端结肠的肠细胞标记可观察到深至腮红边缘。在远端结肠，中隐窝细胞顶端可见染色。在皮肤和食道中，鳞状上皮的内层有斑点状免疫反应。在胆小管深处的肝细胞中有明显的Rab11免疫反应。胰腺腺泡细胞点状染色。在集合管细胞和前列腺腺细胞中也观察到顶端染色。这些结果表明，Rab11在离散上皮细胞群的顶端囊泡群中表达。
Rab proteins are involved in many aspects of dynamic vesicle processing within eukaryotic cells. We have previously identified Rab11 in gastric parietal cell tubulovesicle membranes. We have produced a monoclonal antibody that is specific for Rab11. In all rabbit tissues examined, Rab11 immunoreactivity was highly enriched in epithelial cells. In the gastric fundus, parietal cells were stained in a pattern consistent with localization on tubulovesicles. Surface mucous cells of both the fundus and antrum demonstrated punctate subapical staining. Ileal and proximal colonic enterocyte labeling was observed deep to the blush borders. In the distal colon, staining was observed in the apical regions of mid-crypt cells. In skin and esophagus, punctate immunoreactivity was present in the medial layers of the squamous epithelia. Prominent Rab11 immunoreactivity was present in hepatocytes deep to the bile canaliculi. Punctate subapical staining was observed in pancreatic acinar cells. Apical staining was also observed in collecting duct cells and in the glandular cells of the prostate. These results indicate that Rab11 is expressed in apical vesicular populations in discrete epithelial cell populations.