METABOLIC-ACTIVATION OF 7,12-DIMETHYLBENZ[A]ANTHRACENE IN RAT MAMMARY TISSUE - FLUORESCENCE SPECTRAL CHARACTERISTICS OF HYDROCARBON-DNA ADDUCTS
METABOLIC-ACTIVATION OF 7,12-DIMETHYLBENZ[A]ANTHRACENE IN RAT MAMMARY TISSUE - FLUORESCENCE SPECTRAL CHARACTERISTICS OF HYDROCARBON-DNA ADDUCTS
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DOI：
10.1016/0304-3835(85)90172-7
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发表时间：
1985-01-01
期刊：
影响因子：
9.7
通讯作者：
SIMS, P
中科院分区：
文献类型：
--
作者：
VIGNY, P;BRUNISSEN, A;SIMS, P
存在于从用7，12-二甲基-苯并[α]蒽（DMBA）经Sephadex LH 20柱层析分离，高效液相色谱（HPLC）纯化，光子计数荧光光谱法测定。发现加合物具有类似蒽的荧光光谱，这与DMBA 1，2，3，4-环上形成的二醇环氧化物与乳腺DNA的反应一致。
The hydrocarbon deoxyribonucleoside adducts present in DNA isolated from the mammary glands of rats that had been treated with 7,12-dimethyl-benz[.alpha.]anthracene (DMBA) were separated by Sephadex LH20 column chromatography, purified by high performance liquid chromatography (HPLC) and examined by photon-counting spectrophotofluorimetry. The adducts were found to have anthracene-like fluorescence spectra which is consistent with the reaction of diol-epoxides formed in the 1,2,3,4-ring of DMBA with mammary gland DNA.