Early seizure detection in rats based on vagus nerve activity
Early seizure detection in rats based on vagus nerve activity
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DOI：
10.1007/s11517-010-0683-1
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发表时间：
2011-02-01
影响因子：
3.2
通讯作者：
Struijk, Johannes J.
中科院分区：
文献类型：
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作者：
Harreby, Kristian R.;Sevcencu, Cristian;Struijk, Johannes J.
持续、预定的迷走神经刺激（VNS）用于治疗难治性癫痫。在癫痫发作之前或发作时开始的按需VNS可以改善治疗效果，然而，这需要早期预测或检测癫痫发作。本研究旨在探讨基于颈部迷走神经电图（VENG）的早期癫痫发作检测的可能性。14只麻醉大鼠接受生理盐水（对照组，n = 6）或稀释于生理盐水中的戊四唑（PTZ）（PTZ治疗组，n = 8）静脉输注（IV）。心脏相关VENG曲线（CrVENG）是通过使用VENG能量的R峰触发平均值得出的。随后，将该特征的变化作为癫痫发作预测因子进行评价。使用左神经VENG，癫痫发作检测在所有PTZ治疗的大鼠103 +/- A 51 s（平均值+/- A SD）之前，他们开发强直性癫痫发作。对照组大鼠没有出现癫痫发作，我们的方法也没有在这些大鼠中检测到癫痫发作。麻醉大鼠左侧神经VENG可早期发现癫痫发作。发作前CrVENG变化可能反映中枢介导的变化和/或呼吸与心动周期之间关系的变化。还需要进一步的研究来评估这种方法在清醒和自由活动的动物中的效果，并最终在人类中进行评估。
Continuous, scheduled vagus nerve stimulation (VNS) is used for the treatment of refractory epilepsy. On-demand VNS, started prior to or at the onset of a seizure may improve the effect of the treatment, however, this requires seizures to be predicted or detected early. This study investigates the possibility of early seizure detection based on the cervical vagus electroneurogram (VENG). Fourteen anesthetized rats received an intravenous infusion (IV) of either saline (control, n = 6) or pentylenetetrazol (PTZ) diluted in saline (PTZ-treated, n = 8). A cardiac-related VENG profile (CrVENG) was derived by using R-peak triggered averaging of the VENG energy. Following, changes in this profile were evaluated as a seizure predictor. Using left nerve VENG, seizures were detected in all PTZ-treated rats 103 +/- A 51 s (mean +/- A SD) before they developed tonic seizures. Control rats did not develop seizures and our method did also not detected seizures in these rats. Seizures can be early detecting based on left nerve VENG in anesthetized rats. Preictal CrVENG changes may reflect central-mediated changes and/or changes in the relation between the respiration and the cardiac cycle. Further research is needed to evaluate the method in awake and freely moving animals and eventually in humans.