Effects of lycopene on skeletal muscle-fiber type and high-fat diet-induced oxidative stress
Effects of lycopene on skeletal muscle-fiber type and high-fat diet-induced oxidative stress
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DOI：
10.1016/j.jnutbio.2020.108523
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发表时间：
2021-01-01
影响因子：
5.6
通讯作者：
Zhou, Lei
中科院分区：
文献类型：
--
作者：
Liu, Siqi;Yang, Dan;Zhou, Lei
越来越多的研究表明，许多天然产物可以参与肌纤维的形成。本研究旨在探讨番茄红素对体内外骨骼肌纤维类型的影响。体外研究中使用C2 C12成肌细胞，番茄红素的浓度为10 μ M。在体内，使用8周龄的雄性C57/BL 6小鼠并将其分为四组（n = 8）：（1）ND：正常脂肪饮食;（2）ND + Lyc：与0.33%w/w番茄红素混合的正常脂肪饮食;（3）HFD：高脂肪饮食;和（4）HFD + Lyc：与0.33%w/w番茄红素混合的高脂肪饮食。喂养8周后收集小鼠组织样品，发现番茄红素的补充提高了慢缩纤维蛋白的表达，琥珀酸脱氢酶和苹果酸脱氢酶的活性，而番茄红素降低了快缩纤维蛋白的表达，乳酸脱氢酶和丙酮酸激酶的活性。此外，番茄红素还能促进骨骼肌甘油三酯沉积，增强脂质合成相关基因的mRNA表达，降低脂解相关基因的mRNA表达。补充番茄红素后，高脂饮食引起的血脂异常和氧化应激均有所减轻。另外，补充番茄红素降低了C2 C12细胞的糖酵解储备，但增加了线粒体ATP的产生，这些结果表明番茄红素影响肌纤维中代谢酶的活性，促进慢收缩纤维的表达，增强线粒体呼吸能力。我们推测番茄红素通过有氧氧化作用影响肌纤维类型，这表明番茄红素对骨骼肌代谢具有潜在的有益作用。(C)2020爱思唯尔公司All rights reserved.
Increasing studies report that many natural products can participate in formation of muscle fibers. This study aimed to investigate the effect of lycopene on skeletal muscle-fiber type in vivo and in vitro . C2C12 myoblasts were used in vitro study, and the concentration of lycopene was 10 mu M. In vivo , 8-week-old male C57/BL6 mice were used and divided into four groups (n = 8): (1) ND: normal-fat diet; (2) ND + Lyc: normal-fat diet mixed with 0.33% w/w lycopene; (3) HFD: high-fat diet; and (4) HFD + Lyc: high-fat diet mixed with 0.33% w/w lycopene. The mice tissue samples were collected after 8 weeks feeding.We found that lycopene supplementation enhanced the protein expression of slow-twitch fiber, succinate dehydrogenase, and malic dehydrogenase enzyme activities, whereas lycopene reduced the protein expression of fast-twitch fibers, lactate dehydrogenase, pyruvate kinase enzyme activities. Moreover, lycopene can promote skeletal muscle triglyceride deposition, enhanced the mRNA expression of genes related to lipid synthesis, reduced the mRNA expression of genes related to lipolysis. And high-fat diet-induced dyslipidemia and oxidative stress were attenuated after lycopene supplementation. Additionally, lycopene supplementation reduced the glycolytic reserve but enhanced mitochondrial ATP production in C2C12 cells.These results demonstrated that lycopene affects the activities of metabolic enzymes in muscle fibers, promotes the expression of slow-twitch fibers, and enhanced mitochondrial respiratory capacity. We speculated that lycopene affects the muscle-fiber type through aerobic oxidation, suggesting that lycopene exerts potential beneficial effects on skeletal muscle metabolism. (C) 2020 Elsevier Inc. All rights reserved.