Gdf6 induces commitment of pluripotent mesenchymal C3H10T1/2 cells to the adipocyte lineage
Gdf6 induces commitment of pluripotent mesenchymal C3H10T1/2 cells to the adipocyte lineage
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DOI：
10.1111/febs.12256
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发表时间：
2013-06
期刊：
影响因子：
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通讯作者：
Shan‐Shan Wang;Haiyan Huang;Su-Zhen Chen;Xi Li;Wenting Zhang;Q. Tang
中科院分区：
文献类型：
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作者：
Shan‐Shan Wang;Haiyan Huang;Su-Zhen Chen;Xi Li;Wenting Zhang;Q. Tang
间充质干细胞具有分化成多种细胞类型的潜能，包括成骨细胞、软骨细胞、肌细胞和脂肪细胞。生长分化因子6 （Gdf6）是转化生长因子β超家族的成员。我们研究了Gdf6在间充质干细胞脂肪形成中的潜在作用，并发现Gdf6的过表达诱导多能间充质C3H10T1/2细胞向脂肪细胞系的转变。I型受体Bmpr1a和II型受体Bmpr2和Acvr2a介导Gdf6信号通路。RNAi对Smad4和p38 MAPK的沉默表明Smad和p38 MAPK通路都参与了这一过程。Runx1t1的表达在承诺的前脂肪细胞中下调，并且Runx1t1的强制表达阻断了脂肪细胞的承诺。结果表明Gdf6在脂肪细胞的承诺和分化中发挥作用。
Mesenchymal stem cells have the potential to undergo commitment and differentiation into a variety of cell types, including osteoblasts, chondrocytes, myocytes and adipocytes. Growth differentiation factor 6 (Gdf6) is a member of the transforming growth factor β superfamily. We have examined the potential role of Gdf6 in adipogenesis of mesenchymal stem cells, and found that over‐expression of Gdf6 induced commitment of pluripotent mesenchymal C3H10T1/2 cells to the adipocyte lineage. The type I receptor Bmpr1a and the type II receptors Bmpr2 and Acvr2a mediate the Gdf6 signaling pathway. RNAi silencing of Smad4 and p38 MAPK suggested that both Smad and p38 MAPK pathways are involved in this process. The expression of Runx1t1 was down‐regulated in committed pre‐adipocytes, and forced expression of Runx1t1 blocked the adipocytic commitment. The results demonstrate a role for Gdf6 in adipocytic commitment and differentiation.