Proteomic analysis of Chelidonium majus milky sap using two-dimensional gel electrophoresis and tandem mass spectrometry
Proteomic analysis of Chelidonium majus milky sap using two-dimensional gel electrophoresis and tandem mass spectrometry
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DOI：
10.1016/j.phytochem.2007.03.039
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发表时间：
2007-06-01
期刊：
影响因子：
3.8
通讯作者：
Gozdzicka-Jozefiak, Anna
中科院分区：
文献类型：
--
作者：
Nawrot, Robert;Kalinowski, Andrzej;Gozdzicka-Jozefiak, Anna
乳状液，一种乳状的橙子液体，从白屈菜中分离出来，罂粟科植物，作为各种生物活性物质的丰富来源，如生物碱，几种黄酮类化合物，酚酸和蛋白质。采用双向凝胶电泳（2-DE）技术分离青竹乳汁提取物中的蛋白质组分，并利用液相色谱-串联质谱（LC-ESI-MS/MS）技术对其进行鉴定。可以鉴定出21种蛋白质，其中包括疾病/防御相关蛋白质、信号传导蛋白质、克雷布斯循环蛋白质、核酸结合蛋白质和其他蛋白质。大多数已鉴定的蛋白质可以与直接和间接的应激和防御反应相关联，例如针对不同病原体的反应。乳状液的特定蛋白质组成表明这些蛋白质对整个植物的生理和发育具有重要作用。(c)2007爱思唯尔有限公司保留所有权利。
Milky sap, a milky-like orange fluid, isolated from the Greater Celandine (Chelidonium majus L.), family Papaveraceae, serves as a rich source of various biologically active substances such as alkaloids, several flavonoids, phenolic acids and proteins. The objective of this study was to separate Ch. majus milky sap extract proteins using two-dimensional gel electrophoresis (2-DE) to demonstrate for the first time the protein composition in the sap and to identify them using liquid chromatography-tandem mass spectrometry analysis (LC-ESI-MS/MS). It was possible to identify 21 proteins, which comprise disease/defence-related, signalling, Krebs cycle, nucleic acid binding and other proteins. The majority of the identified proteins can be linked to direct and indirect stress and defence reactions, e.g. against different pathogens. The specific protein composition of the milky sap suggests an important role of these proteins for the whole plant physiology and development. (c) 2007 Elsevier Ltd. All rights reserved.