Paraferrimonas haliotis sp nov., isolated from the intestine of abalone, Haliotis discus hannai and emendation of description of the genus Paraferrimonas
Paraferrimonas haliotis sp nov., isolated from the intestine of abalone, Haliotis discus hannai and emendation of description of the genus Paraferrimonas
复制标题
Paraferrimonas haliotis sp nov.，从鲍鱼、皱纹盘鲍的肠道中分离出来，并修正了 Paraferrimonas 属的描述
DOI：
10.1099/ijsem.0.002414
复制
发表时间：
2017
影响因子：
2.8
通讯作者：
Shao Zongze
中科院分区：
文献类型：
--
作者：
Huang Jingran;Huang Zhaobin;Shao Zongze
从皱纹盘鲍肠道分离到两株菌株AFRC7-2-1T和CFD7-4-2。细菌细胞革兰氏阴性，呈棒状，无鞭毛。它们是兼性厌氧细菌，能将Fe(III)还原为Fe(II)。两个菌株的16SRRNA基因序列相似性为99.7 %，其中与副单胞菌NBRC 101628T的相似性最高，为97.3-97.5 %，与其他种的相似性最高，为94.0 %。亲缘关系分析表明，两株菌与一株副病原单胞菌形成了紧密的分支。CGB11是从鲍鱼、杂色鲍鱼和鲍鱼肠道中提取的。SeDimticolaNBRC 101628T结果表明，菌株AFRC7-2-1T和CFD7-4-2的DNA-DNA杂交(DDH)值与P.SeDimticolaNBRC101628T为18.8~18.9 %。AFRC7-2-1T和CFD7-4-2的平均核苷酸同源性(ANI)值与P.SeDimticolaNBRC101628T为71.3~71.5 %。AFRC7-2-1T和CFD7-4-2菌株的主要脂肪酸(>；5 %)分别为异C15 ： 0、异C13 ： 0、C14 ： 0、C16 ： 0、C17 ： 1ω8c和总和特征3(C16 ： 1ω7c/C16 ： 1ω6c)。菌株AFRC7-2-1T和CFD7-4-2的对苯二酚体系为：对苯二酚-8、对苯二酚-6、对苯二酚-7和对苯二酚-7。菌株AFRC7-2-1T的主要极性脂类为磷脂酰乙醇胺(PE)、磷脂酰甘油(PG)、1种未鉴定的磷脂(PL)和3种次要的未鉴定脂(LS)。两株病毒基因组G+C- 含量分别为47.2mol%和47.2mol%。综上所述，这两个菌株代表了副单胞菌属的一个新种，命名为盐生副单胞菌。11月模式菌株AFRC7-2-1T(=MCCC1A11748T=KCTC52632T=NBRC 112785T)和CFD7-4-2(=MCCC1A11749=KCTC 52631=NBRC 112786)。
Two strains, AFRC7-2-1Tand CFD7-4-2, were isolated from the intestine of abalone,Haliotis discus hannai. The bacterial cells were Gram-stain-negative, stick-shaped and non-flagellated. They were facultative anaerobic bacteria and could reduce Fe(III) to Fe(II). The 16S rRNA genes of two strains shared 99.7 % sequence similarity, and had the highest similarity of 97.3–97.5 % withParaferrimonas sedimenticolaNBRC 101628T, and of <94.0 % with other species. Phylogenetic relationship showed that the two strains formed a tight clade with an isolateParaferrimonassp. CGB11 obtained from the intestine of small abalone,H. diversicolor, andP. sedimenticolaNBRC 101628T. The resulted DNA–DNA hybridization (DDH) values of strains AFRC7-2-1Tand CFD7-4-2 compared withP. sedimenticolaNBRC 101628Twas 18.8–18.9 %. The resultant average nucleotide identity (ANI) values of AFRC7-2-1Tand CFD7-4-2 compared withP. sedimenticolaNBRC 101628Twas 71.3–71.5 %. The predominant fatty acids (>5 %) of strains AFRC7-2-1Tand CFD7-4-2 were iso-C15 : 0, iso-C13 : 0, C14 : 0, C16 : 0, C17 : 1ω8cand summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The quinone system of strains AFRC7-2-1Tand CFD7-4-2 were quinone-8, menaquinone-6, menaquinone-7, and quinone-7. The predominant polar lipids of strain AFRC7-2-1Twere identified as phosphatidylethanolamine (PE), phosphatidylglycerol (PG), one unidentified phospholipid (PL), and three minor unidentified lipids (Ls). The genomic DNA G+C contents of two strains were 47.2 mol%. In summary, the two strains represented a novel species of the genusParaferrimonas, for which the nameParaferrimonas haliotissp. nov. was proposed, with type strain AFRC7-2-1T(=MCCC 1A11748T=KCTC 52632T=NBRC 112785T) and strain CFD7-4-2 (=MCCC 1A11749=KCTC 52631=NBRC 112786).