EXPRESSION OF CONNEXIN 35/36 IN RETINAL HORIZONTAL AND BIPOLAR CELLS OF CARP
EXPRESSION OF CONNEXIN 35/36 IN RETINAL HORIZONTAL AND BIPOLAR CELLS OF CARP
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CONNEXIN 35/36 在 CARP 视网膜水平和双极细胞中的表达
DOI：
10.1016/j.neuroscience.2009.09.035
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发表时间：
2009-12-15
期刊：
影响因子：
3.3
通讯作者：
Yang, X. L.
中科院分区：
文献类型：
--
作者：
Liu, C. R.;Xu, L.;Yang, X. L.
连接蛋白35/36（Cx 35/36）间隙连接蛋白在包括视网膜在内的脑的各个区域中表达。本研究采用免疫细胞化学方法研究了Cx 35/36在鲤鱼视网膜外层的表达。通过光学显微镜，观察到较强的点状Cx 35/36免疫反应在外丛状层。视网膜垂直切片的双标记实验表明，Cx 35/36斑点位于锥体蒂下方，用恢复素染色，而不是在锥体蒂上。此外，在视网膜切片中，少数由PKC α染色的杆状显性ON型双极细胞（rod-ON-BCs）的树突用Cx 35/36标记。在分离的细胞标本中，Cx 35/36在视锥-显性ON型双极细胞（cone-ON-BCs）的树突上清楚地表达，而在视杆-ON-BCs上表达少得多。此外，Cx 35/36斑点被发现在孤立的水平细胞（标记为GAD 65/67）驱动的锥，包括H1和H2细胞的树突，而不是在那些由杆驱动的细胞（H4细胞）。在超微结构水平上，H1和H2细胞树突内陷视锥终末中发现反应产物，而H4细胞树突内陷视杆终末中未发现反应产物。此外，还标记了锥体-ON-BC的树突。这些结果表明，Cx 35/36可能特异性地参与了鲤鱼视网膜外膜视锥细胞信号通路的调控。(C)2009年IBRO。由Elsevier Ltd.出版。保留所有权利。
Connexin 35/36 (Cx35/36) gap junction protein is expressed in various regions of the brain, including the retina. In this work, the expression of Cx35/36 in the outer retina of carp was studied by immunocytochemistry. By light microscopy, strong punctate Cx35/36-immunoreactivity was observed in the outer plexiform layer. Double labeling experiments on vertical retinal sections showed that Cx35/36 puncta were localized beneath cone pedicles, stained by recoverin, but not on them. In addition, few of the dendrites of rod-dominant ON type bipolar cells (rod-ON-BCs), stained by PKC alpha, were labelled with Cx35/36 in the retinal sections. In isolated cell preparations, Cx35/36 was clearly expressed on the dendrites of cone-dominant ON type bipolar cells (cone-ON-BCs), but the expression was much less on rod-ON-BCs. Moreover, Cx35/36 puncta were found in the dendrites of isolated horizontal cells (labelled by GAD 65/67) driven by cones, including H1 and H2 cells, but not in those of cells driven by rods (H4 cells). At the ultrastructural level, reaction product was found in H1 and H2 cell dendrites invaginating cone terminals, but not in H4 cell dendrites invaginating rod terminals. Moreover, dendrites of cone-ON-BCs, were also labebed. These results suggest that Cx35/36 could be specifically involved in modulation of the cone signal pathway in the outer retina of carp. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.