Frequency of glucose-6-phosphate dehydrogenase-deficient red blood cell units in a metropolitan transfusion service
Frequency of glucose-6-phosphate dehydrogenase-deficient red blood cell units in a metropolitan transfusion service
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DOI：
10.1111/j.1537-2995.2012.03765.x
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发表时间：
2013-03-01
期刊：
影响因子：
2.9
通讯作者：
Spitalnik, Steven L.
中科院分区：
文献类型：
--
作者：
Francis, Richard O.;Jhang, Jeffrey;Spitalnik, Steven L.
背景：葡萄糖-6-磷酸脱氢酶（G6 PD）缺乏的特征是红细胞（RBC）在氧化应激反应中的破坏。虽然献血者没有进行常规的G6 PD缺乏症筛查，但输注储存的G6 PD缺陷型红细胞可能会产生严重的不良后果。通过测量来自大都市医院输血服务的RBC单位的G6 PD酶活性，我们试图确定1）G6 PD缺陷RBC单位的患病率，2）储存期间G6 PD活性是否变化，以及3）片段中的G6 PD活性是否与其在袋中的活性相关。研究设计和方法：在301个随机选择的RBC单位和73个D+CE（即，R 0 r或R 0 R 0）RBC单位，均储存在添加剂溶液中。G6 PD缺乏定义为活性低于正常均值的60%。结果：一般调查中G6 PD缺陷单位的频率为0.3%（1/301; 95%置信区间[CI]，
BACKGROUND: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is characterized by red blood cell (RBC) destruction in response to oxidative stress. Although blood donors are not routinely screened for G6PD deficiency, the transfusion of stored G6PD-deficient RBCs may have serious adverse outcomes. By measuring G6PD enzyme activity of RBC units from a large metropolitan hospital transfusion service, we sought to determine 1) the prevalence of G6PD-deficient RBC units, 2) if G6PD activity changes during storage, and 3) if G6PD activity in segments correlates with its activity in the bags. STUDY DESIGN AND METHODS: Quantitative G6PD activity was measured in 301 randomly selected RBC units and 73 D+CE (i.e., R0r or R0R0) RBC units, all stored in additive solutions. G6PD deficiency was defined as activity less than 60% of the normal mean. RESULTS: The frequency of G6PD-deficient units in the general inventory was 0.3% (1/301; 95% confidence interval [CI],