Bex1 knock out mice show altered skeletal muscle regeneration
Bex1 knock out mice show altered skeletal muscle regeneration
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DOI：
10.1016/j.bbrc.2007.08.186
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发表时间：
2007-11-16
影响因子：
3.1
通讯作者：
Margolis, Frank L.
中科院分区：
文献类型：
--
作者：
Koo, Jae Hyung;Smiley, Mark A.;Margolis, Frank L.
Bex1和钙调蛋白（CaM）在骨骼肌再生过程中上调。我们证实了这一发现，并证明了它们以钙依赖的方式相互作用的新发现。为了研究Bex1在骨骼肌再生中的作用及其与CaM的相互作用，我们制造了Bex1敲除（Bex1- ko）小鼠。这些小鼠似乎发育正常且具有生育能力，但与野生型（WT）小鼠相比，在运动表现方面表现出功能性缺陷。在肌内注射心脏毒素后，与WT小鼠相比，Bex1-KO小鼠的再生肌肉表现出细胞增殖升高和延长，细胞分化延迟。心脏毒素会引起广泛和可重复的肌肉损伤，随后恢复。因此，我们的研究结果提供了Bex1- ko小鼠显示肌肉再生改变的第一个证据，并允许我们提出Bex1与Ca2+/CaM的相互作用可能参与骨骼肌再生。(C) 2007爱思唯尔公司版权所有。
Bex1 and Calmodulin (CaM) are upregulated during skeletal muscle regeneration. We confirm this finding and demonstrate the novel finding that they interact in a calcium-dependent manner. To study the role of Bex1 and its interaction with CaM in skeletal muscle regeneration, we generated Bex1 knock out (Bex1-KO) mice. These mice appeared to develop normally and are fertile, but displayed a functional deficit in exercise performance compared to wild type (WT) mice. After intramuscular injection of cardiotoxin, which causes extensive and reproducible myotrauma followed by recovery, regenerating muscles of Bex1-KO mice exhibited elevated and prolonged cell proliferation, as well as delayed cell differentiation, compared to WT mice. Thus, our results provide the first evidence that Bex1-KO mice show altered muscle regeneration, and allow us to propose that the interaction of Bex1 with Ca2+/CaM may be involved in skeletal muscle regeneration. (C) 2007 Elsevier Inc. All rights reserved.