Two new prenylflavonoids from Epimedii Herba and their inhibitory effects on advanced glycation end-products
Two new prenylflavonoids from Epimedii Herba and their inhibitory effects on advanced glycation end-products
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DOI：
10.1007/s11418-015-0962-0
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发表时间：
2016-04-01
影响因子：
3.3
通讯作者：
Ikeda, Tsuyoshi
中科院分区：
文献类型：
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作者：
Nakashima, Keisuke;Miyashita, Hiroyuki;Ikeda, Tsuyoshi
由于晚期糖基化终末产物(AGEs)的抑制剂，如吡哆胺，可显著抑制链脲佐菌素诱导的糖尿病大鼠视网膜病变和神经病变的发展，因此AGE抑制剂被认为是预防糖尿病并发症等生活方式相关疾病的潜在策略。本文研究了淫羊藿醇提物(EH；Epimedium spp.)发现在胶原明胶与核糖孵育过程中，能抑制N(Epsilon)-(羧甲基)赖氨酸(CML)和N(Omega)-(羧甲基)精氨酸(CMA)的形成。此外，从EH中分离得到了抑制CML和CMA形成的化合物。两个新的戊烯基黄酮类化合物(化合物1和2)和两个已知的化合物(化合物3和4)对CML和CMA的形成都有明显的抑制作用，化合物4(淫羊藿素B)的抑制作用最强。这些数据表明，淫羊藿素B可以通过抑制AGEs来预防糖尿病的临床并发症。
Because inhibitors of advanced glycation end-products (AGEs), for example pyridoxamine, significantly inhibit the development of retinopathy and neuropathy in rats with streptozotocin-induced diabetes, treatment with AGE inhibitors is believed to be a potential strategy for the prevention of lifestyle-related diseases such as diabetic complications. In the present study, the MeOH extract of Epimedii Herba (EH; aerial parts of Epimedium spp.) was found to inhibit the formation of N (epsilon) -(carboxymethyl)lysine (CML) and N (omega) -(carboxymethyl)arginine (CMA) during incubation of collagen-derived gelatin with ribose. Furthermore, compounds with inhibitory effects against CML and CMA formation were isolated from EH. Two new prenylflavonoids (compounds 1 and 2) and two known compounds (3 and 4) were found to significantly inhibit the formation of both CML and CMA; compound 4 (epimedokoreanin B) had the strongest inhibitory effect of the isolated compounds. These data suggest that epimedokoreanin B could prevent clinical complications of diabetes by inhibiting AGEs.