RAPID METHODS FOR ISOLATION OF HUMAN-PLASMA FIBRONECTIN
RAPID METHODS FOR ISOLATION OF HUMAN-PLASMA FIBRONECTIN
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DOI：
10.1016/0049-3848(82)90272-9
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发表时间：
1982-01-01
影响因子：
7.5
通讯作者：
MENACHE, D
中科院分区：
文献类型：
--
作者：
MIEKKA, SI;INGHAM, KC;MENACHE, D
建立了一种简便的明胶-琼脂糖亲和层析法分离人血浆纤维连接蛋白的方法。在1种方法中，用3 M尿素洗脱纤连蛋白，并通过将蛋白吸附到肝素琼脂糖上，然后用0.4 M NaCl洗脱来快速去除该试剂。在一个较短的过程中，纤连蛋白从明胶-琼脂糖洗脱简单地通过降低缓冲液pH值低于6。冻干后，纯化的蛋白质容易溶于水中。未吸附到明胶的馏分用于纯化其他蛋白质，包括因子VIII，其促凝血活性被定量回收。
Simplified procedures were developed for isolation of human plasma fibronectin by affinity chromatography on gelatin-agarose. In 1 method, fibronectin was eluted with 3 M urea and this reagent was quickly removed by adsorbing the protein onto heparinagarose, followed by 0.4 M NaCl elution. In a shorter process, fibronectin was eluted from gelatin-agarose simply by decreasing the buffer pH below 6. After lyophilization, the purified protein was readily dissolved in water. The fraction not adsorbed to gelatin was used to purify other proteins, including factor VIII whose procoagulant activity was quantitatively recovered.