ESTERASES IN HUMAN LEUKOCYTES
ESTERASES IN HUMAN LEUKOCYTES
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人类白细胞中的酯酶
DOI：
10.1177/21.1.1
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发表时间：
1973
影响因子：
3.2
通讯作者：
K. Lam
中科院分区：
文献类型：
--
作者：
C. Li;L. Yam;K. Lam
用细胞化学方法和聚丙烯酰胺凝胶电泳法对人白细胞的酯酶进行了研究。本研究的目的是明确各种细胞化学方法的特异性，并建立特异性的方法来显示血细胞中各种类型的酯酶。在pH 4.0条件下，聚丙烯酰胺凝胶电泳可分离出9条同工酶条带。条带1、2、7、8和9在萘酚as - d氯乙酸中表现得最好，并且通过细胞化学方法在粒细胞中证实了条带1、2、7、8和9代表氯乙酸酯酶的活性。带3、4、5和6在萘酯中表现最好，在单核细胞、血小板和浆细胞中表现为“非特异性酯酶”。“氨基己酸酯酶”或“胰蛋白酶样酶”仅在人类肥大细胞中被证实。它在凝胶系统中的确切位置是未知的，因为纯人类肥大细胞制备无法用于研究。这三组酯酶具有相当的底物特异性，但不是绝对的。只有在适当控制的染色条件下，它们才能在各种类型的人类血细胞中特异性地表现出来。
The esterases of human leukocytes were studied both by cytochemical methods and by polyacrylamide gel electrophoresis. The aim of the study was to clarify the specificity of various cytochemical methods and to establish specific methods for demonstrating the various types of esterases in the blood cells. Nine bands of isoenzymes were separable by polyacrylamide gel electrophoresis at pH 4.0. Bands 1, 2, 7, 8 and 9 were best demonstrated by naphthol AS-D chloroacetate and represented the activity of chloroacetate esterase as demonstrated by cytochemical methods in the granulocytes. Bands 3, 4, 5 and 6 were best demonstrated by naphthyl esters and represented "nonspecific esterases" as demonstrated by cytochemical methods in the monocytes, platelets and plasma cells. "Aminocaproate esterase" or "trypsin-like enzyme" was demonstrated only in the human mast cells. Its exact location in the gel system is unknown since pure human mast cell preparation was not available for study. These three groups of esterases had a substantial but not absolute substrate specificity. They can be specifically demonstrated in the various types of human blood cells only under properly controlled staining conditions.