Epithelial cell kinetics in the inflammatory process of chicken trachea infected with infectious bronchitis virus
Epithelial cell kinetics in the inflammatory process of chicken trachea infected with infectious bronchitis virus
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DOI：
10.1292/jvms.62.129
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发表时间：
2000-02-01
影响因子：
1.2
通讯作者：
Gohda, M
中科院分区：
文献类型：
--
作者：
Kotani, T;Shiraishi, Y;Gohda, M
本文对鸡气管内接种传染性支气管炎病毒（IBV）后，气管上皮细胞变性和再生的各个阶段进行了形态学研究。在接种后1至7天（d.p.i.），在气管上皮细胞的细胞质中检测到病毒抗原。峰值在3d.p.i.在1 dpi时，几乎所有的上皮细胞都参与了变性。在这个时候基底细胞的BrdU标记指数显著高于对照组。在2和3 d.p.i.。基底细胞数目较少，但BrdU标记指数有下降趋势。在4和5 d.p.i.，基底细胞的BrdU标记指数显著低于1d.p.i.，少量再生的未成熟纤毛上皮细胞出现。注射后6到11天，纤毛柱状上皮细胞数量迅速增加，13 d时除无纤毛斑外基本恢复正常。结果表明，感染IBV后24 h内气管上皮细胞发生变性，基底细胞的增殖活性立即发挥，3 ~ 4d后，基底细胞向纤毛上皮细胞分化。
All stages of degeneration and regeneration in chicken tracheal epithelium were studied morphologically following an intratracheal inoculation of infectious bronchitis virus (IBV). Viral antigen was detected in the cytoplasm of tracheal epithelium from 1 to 7 days post-inoculation (d.p.i.) with a peak on 3 d.p.i. At 1 d.p.i., almost all epithelial cells were involved in the degeneration. At this time. labelling index of bromodeoxyuridine (BrdU) in the basal cells showed significantly high value compared with control. At 2 and 3 d.p.i.. a gnat number of basal cells were recognized, but the BrdU labelling index tended to decrease. At 4 and 5 d.p.i., the BrdU labelling index of basal cells significantly decreased than 1 d.p.i., and a few number of regenerated immature ciliated epithelia appeared. At 6 to 11 d.p.i., the ciliated columnar epithelia increased rapidly in number, and returned to the normal appearance except for non-ciliated patch by 13 d.p.i. These results suggested that the tracheal epithelial cells infected with IBV degenerated within 24 hours and proliferating activity of basal cells functioned immediately, and 3 to 4 days later, these basal cells were differentiated to the ciliated epithelia.