Outer surface protein B is critical for Borrelia burgdorferi adherence and survival within Ixodes ticks
Outer surface protein B is critical for Borrelia burgdorferi adherence and survival within Ixodes ticks
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DOI：
10.1371/journal.ppat.0030033
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发表时间：
2007-03-01
期刊：
影响因子：
6.7
通讯作者：
Fikrig, Erol
中科院分区：
文献类型：
--
作者：
Neelakanta, Girish;Li, Xin;Fikrig, Erol
伯氏疏螺旋体（Borrelia burgdorferi）在蜱虫和哺乳动物中的存活，至少在一定程度上是由脂蛋白的选择性表达促进的。外表面蛋白（Osp） A参与螺旋体附着于蜱虫肠道。由于ospB与ospA在双电子操纵子上表达，我们现在通过产生ospB缺陷的伯氏疏螺旋体并检查其在整个螺旋体生命周期中的表型来研究ospB的作用。与野生型分离株相似，缺乏ospb的伯氏疏螺旋体能够很容易地感染并在小鼠中持续存在。缺乏ospb的伯氏疏螺旋体能够迁移到进食的蜱虫身上，但粘附在蜱虫肠道和在媒介中存活的能力受损。此外，缺乏ospb的伯氏疏螺旋体与蜱虫肠道提取物结合不良。将缺乏ospB基因的螺旋体与ospB基因的野生型拷贝进行互补，恢复了其结合蜱虫肠道的能力。综上所述，这些数据表明OspB在肩胛骨伊蚊中起着重要作用，而伯氏疏螺旋体依靠多种基因有效地在蜱中持续存在。
Survival of Borrelia burgdorferi in ticks and mammals is facilitated, at least in part, by the selective expression of lipoproteins. Outer surface protein (Osp) A participates in spirochete adherence to the tick gut. As ospB is expressed on a bicistronic operon with ospA, we have now investigated the role of OspB by generating an OspB-deficient B. burgdorferi and examining its phenotype throughout the spirochete life cycle. Similar to wild-type isolates, the OspB-deficient B. burgdorferi were able to readily infect and persist in mice. OspB-deficient B. burgdorferi were capable of migrating to the feeding ticks but had an impaired ability to adhere to the tick gut and survive within the vector. Furthermore, the OspB-deficient B. burgdorferi bound poorly to tick gut extracts. The complementation of the OspB-deficient spirochete in trans, with a wild-type copy of ospB gene, restored its ability to bind tick gut. Taken together, these data suggest that OspB has an important role within Ixodes scapularis and that B. burgdorferi relies upon multiple genes to efficiently persist in ticks.