Elongation factor 1 gamma mRNA expression in oesophageal carcinoma
Elongation factor 1 gamma mRNA expression in oesophageal carcinoma
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DOI：
10.1136/gut.38.1.66
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发表时间：
1996-01-01
期刊：
影响因子：
24.5
通讯作者：
Sugimachi, K
中科院分区：
文献类型：
--
作者：
Mimori, K;Mori, M;Sugimachi, K
延伸因子 1 gamma (EF1 gamma) 已知是 EF1 的一个亚基，EF1 是在翻译过程中介导氨酰 tRNA 转运至 80S 核糖体的 G 蛋白之一。由于人们对 EF1 γ 在人类食管癌中的表达知之甚少，因此本研究使用 Northern 印迹分析来观察其表达。对36例食管癌和15个食管癌细胞系进行了研究。与正常组织相比，36 个癌组织中有 5 个 (14%) 的 EF1 gamma mRNA 过度表达水平达到两倍或更高。 5例过表达病例与非过表达病例相比均表现出严重的淋巴结转移，且差异有显着性（p=0.028）。与非过表达病例相比，这5例病例的疾病分期远为晚期（p=0.012）。所有15个食管癌细胞表达的EF1γmRNA相对低于最初分离出EF1γ的胃癌或胰腺癌细胞系。由于即使在活检标本中也能检测到 EF1 γ mRNA 的表达，其在肿瘤组织中的过度表达可能为术前预测肿瘤的侵袭性提供有用的信息。
Elongation factor 1 gamma (EF1 gamma) is known to be a subunit of EF1, one of the G proteins that mediate the transport of aminoacyl tRNA to 80S ribosomes during translation. As Little is known regarding the expression of EF1 gamma in human oesophageal carcinoma, this study looked at its expression using a northern blot analysis. Thirty six cases of oesophageal carcinoma and 15 oesophageal carcinoma cell lines were studied. The EF1 gamma mRNA overexpression at a level of twofold or more was seen in five (14%) of 36 carcinomatous tissues compared with the normal counterparts. All five overexpressed cases showed severe lymph node metastases compared with the non-overexpressed cases, and the difference was significant (p=0.028). The stage of the disease of these five cases was far advanced compared with the non-overexpressed cases (p=0.012). All 15 oesophageal carcinoma cells expressed EF1 gamma mRNA relatively lower than the gastric or pancreatic carcinoma cell lines, in which EF1 gamma was originally isolated. As the expression of EF1 gamma mRNA could be detected even in the biopsy specimens, its overexpression in tumour tissue may provide preoperative useful information for predicting the aggressiveness of tumours.