Overexpression of the acerola (Malpighia glabra) monodehydroascorbate reductase gene in transgenic tobacco plants results in increased ascorbate levels and enhanced tolerance to salt stress
Overexpression of the acerola (Malpighia glabra) monodehydroascorbate reductase gene in transgenic tobacco plants results in increased ascorbate levels and enhanced tolerance to salt stress
复制标题
DOI：
10.1016/j.sajb.2011.08.005
复制
发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Esaka, M.
中科院分区：
文献类型：
--
作者：
Eltelib, H. A.;Fujikawa, Y.;Esaka, M.
单脱氢抗坏血酸还原酶（MDHAR，EC 1.6.5.4）是抗坏血酸（阿萨）-谷胱甘肽循环的关键酶，其维持还原的阿萨库并作为重要的抗氧化酶。以前，我们已经从积累大量阿萨的针叶树（Malpighia glabra）中克隆了MDHAR cDNA。本研究利用农杆菌介导的基因转移系统，将金虎尾MDHAR cDNA导入烟草植株。转基因烟草植株积累了更多的阿萨，表现出更高的MDHAR活性比对照植物。盐胁迫下，转基因植株的膜脂过氧化和叶绿素降解受到抑制，而对照植株的膜脂过氧化和叶绿素降解受到抑制。这些结果表明，针叶树MDHAR的过表达赋予更大的耐盐胁迫。(C)2011款萨博。Elsevier B. V.出版，保留所有权利。
Monodehydroascorbate reductase (MDHAR, EC 1.6.5.4) is a key enzyme of the ascorbate (AsA)-glutathione cycle that maintains reduced pools of AsA and serves as an important antioxidative enzyme. Previously, we have cloned MDHAR cDNA from acerola (Malpighia glabra), a plant that accumulates abundant amount of AsA. In this study, MDHAR cDNA from acerola was introduced into tobacco plants using an Agrobacterium-mediated gene delivery system. Transgenic tobacco plants accumulated greater amounts of AsA and showed higher MDHAR activity than the control plants. Lipid peroxidation and chlorophyll degradation, which were stimulated in control plants, were restrained in transgenic plants subjected to salt stress. These results indicate that overexpression of acerola MDHAR imparts greater tolerance to salt stress. (C) 2011 SAAB. Published by Elsevier B.V. All rights reserved.