Using flotation in ethanol to separate filled and empty seeds of Pinus nigra ssp. pallasiana.
Using flotation in ethanol to separate filled and empty seeds of Pinus nigra ssp. pallasiana.
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使用乙醇浮选法分离黑松的实种子和空种子。
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发表时间：
2010
期刊：
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通讯作者：
M. D. Avşar
中科院分区：
文献类型：
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作者：
M. D. Avşar
本研究对克里米亚松实粒与空粒的分离进行了研究。SSP。Pallasiana(Lamb.)Holmboe]种子萌发情况，并研究了乙醇浮选对种子萌发的影响。采用密度法在96%乙醇中浮选种子，然后对土耳其南部三个克里米亚松种源的种子进行萌发试验。在浮选试验中，97.92%的种子浸泡在乙醇中，98.73%的种子以所有种源的总平均值为充实率。乙醇浮选和对照处理的发芽率分别为98.03%和98.62%，平均发芽势分别为82.12%和79.45%。3个种源的发芽率与对照差异不显著(p>；0.05)，两个种源的发芽率差异不显著(p>；0.05)，其中一个种源的发芽势差异显著(p<；0.05)。研究结果表明，用乙醇浮选法可以较高比例地分离克里米亚松实粒种子和空粒种子，且不会对种子发芽率和发芽势产生负面影响。
In this study, the separation of filled and empty seeds of Crimean pine [Pinus nigra Arn. ssp. pallasiana (Lamb.) Holmboe] by flotation in ethanol and the effect of this treatment on seed germination were investigated. Flotation tests in 96% ethanol by the density method and then germination tests were made on the seeds from three Crimean pine provenances of southern Turkey. In the flotation tests, 97.92% of the filled seeds sank in ethanol and 98.73% of the sunken seeds were filled seeds as the general mean values of all the provenances. Germination percentages of the flotation in ethanol and control treatments were 98.03 and 98.62%, and germination energies were 82.12 and 79.45%, respectively, in the general mean. While there was no statistically significant difference (p > 0.05) between the flotation and control treatments in terms of germination percentage for all three provenances, there was no significant difference (p > 0.05) for two provenances; however, there was a significant difference (p < 0.05) for one provenance in favour of the flotation treatment in terms of germination energy. The results of the study indicated that filled seeds of Crimean pine could be separated from empty seeds at a very high proportion by flotation in ethanol and this treatment did not affect negatively the germination percentage and germination energy of the seeds.