Visualization of cellulose synthase demonstrates functional association with microtubules
Visualization of cellulose synthase demonstrates functional association with microtubules
复制标题
DOI：
10.1126/science.1126551
复制
发表时间：
2006-06-09
期刊：
影响因子：
56.9
通讯作者：
Ehrhardt, David W.
中科院分区：
文献类型：
--
作者：
Paredez, Alexander R.;Somerville, Christopher R.;Ehrhardt, David W.
在转基因拟南芥植物中表达与纤维素合成酶（CESA）融合的功能性黄色荧光蛋白，可以在活细胞中观察纤维素沉积的过程。旋转圆盘共聚焦显微镜显示，CESA复合物在质膜上以恒定的速度移动，线性轨道对齐，并与皮层微管一致。在每个观察到的线性轨道内，复杂的运动是双向的。微管聚合的抑制改变了膜中移动CESA复合物的精细尺度分布和模式，表明细胞骨架指导纤维素沉积的相对直接的机制。
Expression of a functional yellow fluorescent protein fusion to cellulose synthase (CESA) in transgenic Arabidopsis plants allowed the process of cellulose deposition to be visualized in living cells. Spinning disk confocal microscopy revealed that CESA complexes in the plasma membrane moved at constant rates in linear tracks that were aligned and were coincident with cortical microtubules. Within each observed linear track, complex movement was bidirectional. Inhibition of microtubule polymerization changed the fine-scale distribution and pattern of moving CESA complexes in the membrane, indicating a relatively direct mechanism for guidance of cellulose deposition by the cytoskeleton.