Steroidogenesis-stimulating activity in the gonads: comparison of rat testicular fluid with bovine and human ovarian follicular fluids.
Steroidogenesis-stimulating activity in the gonads: comparison of rat testicular fluid with bovine and human ovarian follicular fluids.
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DOI：
10.1095/biolreprod44.5.937
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发表时间：
1991-05
影响因子：
3.6
通讯作者：
M. Hedger;A. Leung;D. Robertson;D. D. de Kretser-D.;G. Risbridger
中科院分区：
文献类型：
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作者：
M. Hedger;A. Leung;D. Robertson;D. D. de Kretser-D.;G. Risbridger
从牛（bFF）和人类（hFF）来源的卵巢卵泡液的类固醇激素生成刺激活性，进行了比较与成年大鼠睾丸液（rTF）使用在体外生物测定系统的基础上刺激睾酮生产纯化的成年大鼠Leydig细胞在20小时的孵育。大鼠TF和bFF进行活性炭处理，以消除类固醇之前的测定，并收集后的凝胶渗透色谱法的hFF的主要活性级分。所有三种液体样品刺激基础和最大hCG刺激的睾酮产生，尽管bFF和hFF提取物的对数剂量-反应线与rTF的对数剂量-反应线不平行。rTF和bFF在相似的剂量范围内（分别为5.2-150微升和9.7-150微升）都有活性，并且都与hCG对睾酮产生的相互作用大于相加作用。在不存在hCG的情况下，hFF提取物的刺激活性显著大于rTF或bFF的刺激活性，但hFF提取物在刺激睾酮产生中仅与hCG具有相加效应。此外，与rTF活性不同，rTF活性通过与蛋白质合成抑制剂放线菌酮共孵育而被抑制，hFF提取物的活性不受放线菌酮的影响。在所有三种液体中负责活性的因子具有大分子大小（大于30 kDa），如通过超滤或凝胶渗透色谱法测定的。然而，与rTF和bFF相反，hFF提取物活性通过活性炭提取去除。人FF提取物被热灭活（100 ℃，30分钟），而rTF活性是部分（70%）热不稳定的，bFF不受心脏的影响。（250字处删节）
The steroidogenesis-stimulating activities of ovarian follicular fluid from bovine (bFF) and human (hFF) sources, were compared with those of adult rat testicular fluid (rTF) using an in vitro bioassay system based on stimulation of testosterone production by purified adult rat Leydig cells during a 20-h incubation. Rat TF and bFF were charcoal-treated to remove steroids prior to assay, and the major active fraction of hFF was collected after gel-permeation chromatography. All three fluid samples stimulated both basal and maximal hCG-stimulated testosterone production, although the resulting log dose-response lines of bFF and the hFF extracts were not parallel with those of rTF. Both rTF and bFF were active over a similar dose range (5.2-150 microliters and 9.7-150 microliters, respectively) and both had a more than additive interaction with hCG on testosterone production. The stimulatory activity of the hFF extract was considerably greater than that of either rTF or bFF in the absence of hCG, but hFF extract had only an additive effect with hCG in stimulating testosterone production. Moreover, unlike rTF activity, which was inhibited by co-incubation with the protein synthesis inhibitor, cycloheximide, the activity of the hFF extract was not affected by cycloheximide. The factors responsible for activity in all three fluids were of a large molecular size (greater than 30 kDa), as determined by ultrafiltration or gel-permeation chromatography. However, in contrast to both rTF and bFF, hFF extract activity was removed by charcoal extraction. Human FF extract was inactivated by heat (100 degrees C, 30 min), whereas rTF activity was partially (70%) heat-labile and bFF was not affected by heart.(ABSTRACT TRUNCATED AT 250 WORDS)