Printed Paper Sensors for Serum Lactate Dehydrogenase using Pullulan-Based Inks to Immobilize Reagents
Printed Paper Sensors for Serum Lactate Dehydrogenase using Pullulan-Based Inks to Immobilize Reagents
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DOI：
10.1021/acs.analchem.5b01923
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发表时间：
2015-09-15
影响因子：
7.4
通讯作者：
Brennan, John D.
中科院分区：
文献类型：
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作者：
Kannan, Balamurali;Jahanshahi-Anbuhi, Sana;Brennan, John D.
在本研究中，开发了一种基于纸质的护理点 (POC) 比色生物传感器，使用基于支链淀粉的无孔、不透氧的可逆胶凝多糖材料来检测血清中的乳酸脱氢酶。普鲁兰多糖可以与所有必需的测定试剂一起印刷到纸表面上，从而提供了一种将所有试剂高稳定性固定在纸上的方法。将含有乳酸脱氢酶（LDH）的血清直接点样到普鲁兰多糖涂覆的生物活性纸上，并提供与传统读板器方法获得的定量比色数据相当的数据。研究发现，该纸条高度稳定，可以在 4 摄氏度下保存至少 10 周，性能不会下降，而在没有稳定多糖的情况下打印试剂时，1 天内性能就完全丧失。易于制造加上印刷试剂的高稳定性为易于制造的 POC 传感器提供了一个简单的平台。
In this study, a paper-based point-of-care (POC) colorimetric biosensor was developed for the detection of lactate dehydrogenase in serum using a nonporous, oxygen impermeable reversibly gelling polysaccharide material based on pullulan. The pullulan could be printed onto paper surfaces along with all required assay reagents, providing a means for high-stability immobilization of all reagents on paper. Serum containing lactate dehydrogenase (LDH) was directly spotted on to the pullulan-coated bioactive paper and provided quantitative colorimetric data that was comparable to that obtained with a conventional plate-reader method. The paper strip was found to be highly stable and could be stored at 4 degrees C for at least 10 weeks with no loss in performance, as compared to a complete loss in performance within 1 day when the reagents were printed without the stabilizing polysaccharide. The ease of fabrication coupled with the high stability of the printed reagents provides a facile platform for easily manufactured POC sensors.