Restricted distribution of mrg-1 mRNA in C. elegans primordial germ cells through germ granule-independent regulation
Restricted distribution of mrg-1 mRNA in C. elegans primordial germ cells through germ granule-independent regulation
复制标题
通过生殖颗粒独立调节限制 mrg-1 mRNA 在秀丽隐杆线虫原始生殖细胞中的分布
作者：
Miwa;T.;Takasaki;T.;Inoue;K.;Sakamoto;H.
染色体结构域蛋白MRG-1是一种重要的母体因子，用于保护秀丽隐杆线虫生殖细胞免于细胞死亡。与从胚胎第一次卵裂开始的每次细胞分裂时仅分离到生殖系卵裂球的生殖颗粒不同，MRG-1在早期胚胎的所有细胞中丰富，然后通过形态发生阶段逐渐限制在原始生殖细胞（PGCs）中。在这里，我们发现这种特征性的时空表达模式是由rg-13'UTR决定的，并且在生殖细胞和体细胞之间在RNA水平上受到差异调节。不对称分离的胚芽颗粒是不必要的本地化MRG-1的PGCs。我们发现，MES-4是生殖细胞中一种重要的染色质调节因子，也以不依赖于生殖颗粒的方式在PGC中积累。我们认为，秀丽隐杆线虫PGCs具有一种新的机制，可以积累至少一些染色质相关蛋白，这些蛋白对于种系永生至关重要。
The chromodomain protein MRG‐1 is an essential maternal factor for proper germline development that protects germ cells from cell death inC. elegans. Unlike germ granules, which are exclusively segregated to the germline blastomeres at each cell division from the first cleavage of the embryo, MRG‐1 is abundant in all cells in early embryos and is then gradually restricted to the primordial germ cells (PGCs) by the morphogenesis stage. Here, we show that this characteristic spatiotemporal expression pattern is dictated by themrg‐13'UTR and is differentially regulated at the RNA level between germline and somatic cells. Asymmetric segregation of germ granules is not necessary to localize MRG‐1 to the PGCs. We found that MES‐4, an essential chromatin regulator in germ cells, also accumulates in the PGCs in a germ granule‐independent manner. We propose thatC. elegansPGCs have a novel mechanism to accumulate at least some chromatin‐associated proteins that are essential for germline immortality.