Single-cell transcriptome conservation in cryopreserved cells and tissues.
Single-cell transcriptome conservation in cryopreserved cells and tissues.
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DOI：
10.1186/s13059-017-1171-9
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发表时间：
2017-03-01
期刊：
影响因子：
12.3
通讯作者：
Heyn H
中科院分区：
文献类型：
--
作者：
Guillaumet-Adkins A;Rodríguez-Esteban G;Mereu E;Mendez-Lago M;Jaitin DA;Villanueva A;Vidal A;Martinez-Marti A;Felip E;Vivancos A;Keren-Shaul H;Heath S;Gut M;Amit I;Gut I;Heyn H
已经有多种单细胞RNA制备流程被描述。到目前为止，相关方案都要求使用新鲜材料，这阻碍了复杂的研究设计。我们描述了一种样本保存方法，该方法能在有活性的单细胞中维持转录本，使得采样的时间和地点能够与后续的处理步骤分离。我们使用3'端和全长RNA制备方法对1000多个新鲜的和冷冻保存的细胞进行单细胞转录组测序。我们的结果证实，保存过程没有改变转录图谱。这极大地拓宽了单细胞转录组学的应用范围，并可能导致未来研究设计的范式转变。
本文的网络版（doi:10.1186/s13059 - 017 - 1171 - 9）包含补充材料，授权用户可获取。
A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs. The online version of this article (doi:10.1186/s13059-017-1171-9) contains supplementary material, which is available to authorized users.