Visualization of microbodies in Chlamydomonas reinhardtii
Visualization of microbodies in Chlamydomonas reinhardtii
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DOI：
10.1007/s10265-011-0469-z
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发表时间：
2012-07-01
影响因子：
2.8
通讯作者：
Shinozaki, Akiko
中科院分区：
文献类型：
--
作者：
Hayashi, Yasuko;Shinozaki, Akiko
在绿藻细胞中，这些细胞器通常被称为微体，因为它们缺乏在高等植物过氧化物酶体中发现的酶。一些藻类中的微体含有的酶比高等植物的过氧化物酶体少，并且绿藻纲中的一些单细胞绿色藻类如莱茵衣藻不具有过氧化氢酶，过氧化氢酶是过氧化物酶体中常见的酶。因此，绿藻中的微体是否类似于高等植物的过氧化物酶体，以及它们是否使用类似的过氧化物酶体靶向信号（PTS）的运输机制，仍然不清楚。为了确定PTS是否存在于绿藻的微体中，并使衣原体细胞中的微体可视化，我们研究了与几种PTS样序列融合的绿色荧光蛋白（GFP）的亚细胞定位。我们在转基因衣原体中检测到直径为0.3-1.0 μ m的球形GFP隔室。通过荧光显微镜观察到的GFP区室的特征和通过电子显微镜观察到的微体的特征的比较分析表明，所述区室是同一个。结果还表明，绿藻细胞中微体的转运机制与高等植物过氧化物酶体的转运机制相似。
In Chlorophycean algal cells, these organelles are generally called microbodies because they lack the enzymes found in the peroxisomes of higher plants. Microbodies in some algae contain fewer enzymes than the peroxisomes of higher plants, and some unicellular green algae in Chlorophyceae such as Chlamydomonas reinhardtii do not possess catalase, an enzyme commonly found in peroxisomes. Thus, whether microbodies in Chlorophycean algae are similar to the peroxisomes of higher plants, and whether they use a similar transport mechanism for the peroxisomal targeting signal (PTS), remain unclear. To determine whether the PTS is present in the microbodies of Chlorophycean algae, and to visualize the microbodies in Chlamydomonas cells, we examined the sub-cellular localization of green fluorescent proteins (GFP) fused to several PTS-like sequences. We detected GFP compartments that were spherical with a diameter of 0.3-1.0 mu m in transgenic Chlamydomonas. Comparative analysis of the character of GFP-compartments observed by fluorescence microscopy and that of microbodies by electron microscopy indicated that the compartments were one and the same. The result also showed that the microbodies in Chlorophycean cells have a similar transport mechanism to that of peroxisomes of higher plants.